Human Collagen alpha-1(XVIII) chain ELISA Kit | MyBioSource MBS2881480 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Collagen alpha-1(XVIII) chain ELISA Kit

catalog :

MBS2881480

quantity :

48-Strip-Wells

price :

380 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2881480

products type :

ELISA Kit

products full name :

Human Collagen alpha-1(XVIII) chain ELISA Kit

products short name :

Collagen alpha-1(XVIII) chain

products name syn :

Collagen alpha-1(XVIII) chain; COL18A1

other names :

collagen alpha-1(XVIII) chain isoform 1 preproprotein; Collagen alpha-1(XVIII) chain; collagen alpha-1(XVIII) chain; collagen type XVIII alpha 1

products gene name :

COL18A1

other gene names :

COL18A1; COL18A1; KS; KNO; KNO1

uniprot entry name :

COIA1_HUMAN

reactivity :

Human

sequence length :

1516

storage stability :

For long term storage, please store the entire kit at -20 degree C.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids

products description :

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

110611235

ncbi acc num :

NP_085059.2

ncbi gb acc num :

NM_030582.3

uniprot acc num :

P39060

ncbi mol weight :

135,761 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (1270258); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (1270247); Collagen Biosynthesis And Modifying Enzymes Pathway (1270246); Collagen Degradation Pathway (1270259); Collagen Formation Pathway (1270245); Degradation Of The Extracellular Matrix Pathway (1270257); Direct P53 Effectors Pathway (137939); Extracellular Matrix Organization Pathway (1270244); FOXA1 Transcription Factor Network Pathway (137979); Integrin Cell Surface Interactions Pathway (1270260)

ncbi summary :

This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

uniprot summary :

COL18A1: COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Defects in COL18A1 are a cause of Knobloch syndrome type 1 (KNO1). An autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele. Belongs to the multiplexin collagen family. 3 isoforms of the human protein are produced by alternative promoter. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 21q22.3. Cellular Component: basement membrane; collagen; endoplasmic reticulum lumen; extracellular matrix; extracellular region; extracellular space. Molecular Function: identical protein binding; metal ion binding; protein binding; structural molecule activity. Biological Process: angiogenesis; cell adhesion; collagen catabolic process; endothelial cell morphogenesis; extracellular matrix disassembly; extracellular matrix organization and biogenesis; negative regulation of cell proliferation; organ morphogenesis; positive regulation of cell migration; positive regulation of cell proliferation; response to drug; response to hydrostatic pressure; visual perception. Disease: Knobloch Syndrome 1

size1 :

48-Strip-Wells

price1 :

380 USD

size2 :

96-Strip-Wells

price2 :

500

more info or order :

MyBioSource product webpage