Mouse Cytosolic phospholipase A2 ELISA Kit | MyBioSource MBS2881394 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Cytosolic phospholipase A2 ELISA Kit

catalog :

MBS2881394

quantity :

48-Strip-Wells

price :

355 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2881394

products type :

ELISA Kit

products full name :

Mouse Cytosolic phospholipase A2 ELISA Kit

products short name :

[Cytosolic phospholipase A2]

products name syn :

[Cytosolic phospholipase A2; cPLA2; Phospholipase A2 group IVA; Pla2g4a; Cpla2; Pla2g4]

other names :

[cytosolic phospholipase A2 isoform 1; Cytosolic phospholipase A2; cytosolic phospholipase A2; phospholipase A2, group IVA (cytosolic, calcium-dependent); Phospholipase A2 group IVAIncluding the following 2 domains:Phospholipase A2 (EC:3.1.1.4)Alternative name(s):Phosphatidylcholine 2-acylhydrolase]

products gene name :

[Pla2g4a]

other gene names :

[Pla2g4a; Pla2g4a; cPLA2; Pla2g4; cPLA2alpha; cPLA2-alpha; Cpla2; Pla2g4; cPLA2]

uniprot entry name :

PA24A_MOUSE

reactivity :

Mouse

sequence length :

748

specificity :

Recombinant and natural Mouse Cytosolic phospholipase A2,cPLA2

storage stability :

For long term storage, please store the entire kit at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: serum, plasma, tissue homogenates?cell culture supernates or other biological fluids. Detection Range: 78-5000 pg/mL

other info2 :

Intra-assay Precision: Intra-Assay CV: ?6.8%. Inter-assay Precision: Inter-Assay CV: ?8.7%

products description :

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates?cell culture supernates or other biological fluids.

ncbi gi num :

6679369

ncbi acc num :

NP_032895.1

ncbi gb acc num :

NM_008869.4

uniprot acc num :

P47713

ncbi mol weight :

85,222 Da

ncbi pathways :

ADP Signalling Through P2Y Purinoceptor 1 Pathway (1323573); Acyl Chain Remodeling Of CL Pathway (1324334); Acyl Chain Remodelling Of PC Pathway (1324325); Acyl Chain Remodelling Of PE Pathway (1324321); Acyl Chain Remodelling Of PG Pathway (1324332); Acyl Chain Remodelling Of PI Pathway (1324330); Acyl Chain Remodelling Of PS Pathway (1324328); Arachidonic Acid Metabolism Pathway (83190); Arachidonic Acid Metabolism Pathway (1324344); Arachidonic Acid Metabolism Pathway (366)

ncbi summary :

The protein encoded by this gene is a member of the phospholipase A2 group IV family. This enzyme hydrolyzes membrane phospholipids, thereby releasing the polyunsaturated fatty acid, arachidonic acid. Arachidonic acid is further metabolized into eicosanoids such as leukotrienes, thromboxanes and prostaglandins, that play important roles in regulating diverse biological processes such as inflammatory responses, membrane and actin dynamics, and tumorigenesis. A rise in intracellular calcium levels results in binding of calcium to the C2 domain of this protein, and triggers the translocation from the cytosol to intracellular membranes, including the Golgi apparatus. Disruption of this gene in mice led to decreased levels of eicosonaoids and platelet-activating factor, decreased allergic symptoms, and impaired reproductive ability in females. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]

uniprot summary :

cPLA2: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Arachidonic acid is a precursor for the eicosanoids that are involved in hemodynamic regulation, inflammatory responses, and other cellular processes. Promotes cerebellar long-term depression and motor learning. Translocates to the Golgi and endoplasmic reticulum in a calcium-dependent fashion. Translocation and activation of at the ER facilitates the process of ER stress. Regulates the biogenesis of lipid droplets, a process dependent on JNK and ceramide kinase. Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+. The N-terminal C2 domain, by its association with lipid membranes, mediates the regulation of CPLA2 by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Inhibited when in trimolecular complex with ANXA2 and S100A10. Phosphorylation of S727 relieves this inhibitory interaction, thus activating PLA2G4A. Protein type: Lipid Metabolism - linoleic acid; Lipid Metabolism - arachidonic acid; Lipid Metabolism - ether lipid; Lipid Metabolism - alpha-linolenic acid; Phospholipase; EC 3.1.1.4; Lipid Metabolism - glycerophospholipid; EC 3.1.1.5. Cellular Component: cytoplasm; cytoplasmic vesicle; cytosol; endoplasmic reticulum; Golgi apparatus; intracellular membrane-bound organelle; nucleus; perinuclear region of cytoplasm. Molecular Function: calcium ion binding; calcium-dependent phospholipase A2 activity; calcium-dependent phospholipid binding; histone acetyltransferase binding; hydrolase activity; lysophospholipase activity; metal ion binding; phospholipase A2 activity; phospholipase activity. Biological Process: arachidonic acid metabolic process; arachidonic acid secretion; decidualization; icosanoid biosynthetic process; lipid catabolic process; lipid metabolic process; metabolic process; ovulation from ovarian follicle; phospholipid catabolic process; positive regulation of apoptosis; positive regulation of cell proliferation; positive regulation of inflammatory response; positive regulation of prostaglandin biosynthetic process; positive regulation of vesicle fusion; regulation of cell proliferation; response to calcium ion; response to organic substance; surfactant homeostasis

size1 :

48-Strip-Wells

price1 :

355 USD

size2 :

96-Strip-Wells

price2 :

475

size3 :

5x96-Strip-Wells

price3 :

2030

size4 :

10x96-Strip-Wells

price4 :

3700

more info or order :

MyBioSource product webpage