catalog number :
MBS2880519
products type :
ELISA Kit
products full name :
Rat C-telopeptide of Collagen alpha-1(II) chain ELISA Kit
products short name :
C-telopeptide of Collagen alpha-1(II) chain
products name syn :
C-telopeptide of Collagen alpha-1(II) chain; CTXII
products gene name :
CTXII
storage stability :
For long term storage, please store the entire kit at -20 degree C.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
products description :
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.