Mouse High mobility group protein B1 (HMGB1) ELISA Kit | MyBioSource MBS287092 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse High mobility group protein B1 (HMGB1) ELISA Kit

catalog :

MBS287092

quantity :

48-Strip-Wells

price :

405 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS287092

products type :

ELISA Kit

products full name :

Mouse High mobility group protein B1 (HMGB1) ELISA Kit

products short name :

[High mobility group protein B1 (HMGB1)]

products name syn :

[DKFZp686A04236; HMG1; HMG3; SBP-1; Amphoterin; OTTHUMP00000018196; OTTHUMP00000190860; OTTHUMP00000200117; Sulfoglucuronyl carbohydrate binding protein; high mobility group box 1; high mobility group prot]

other names :

[high mobility group protein B1; High mobility group protein B1; high mobility group protein B1; high mobility group box 1; High mobility group protein 1; HMG-1]

products gene name :

[HMGB1]

other gene names :

[Hmgb1; Hmgb1; p30; Hmg1; HMG-1; SBP-1; Hmg-1; Hmg1; HMG-1]

reactivity :

Mouse

sequence length :

215

specificity :

This assay has high sensitivity and excellent specificity for detection of Mouse EPI. No significant cross-reactivity or interference between Mouse EPI and analogues was observed.

storage stability :

Store at 2-8 degree C.

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 0.25 ng/mL-50 ng/mL. Sensitivity: 0.1 ng/mL (mean of 6 independent assays).

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: For the quantitative detection of Mouse Epinephrine/Adrenaline (EPI) concentration in serum, plasma and other biological fluids. Principle of the Assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for EPI and Horseradish Peroxidase (HRP) conjugated EPI. The competitive inhibition reaction is launched between with HRP labeled EPI and unlabeled EPI with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of EPI in the sample. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

926657659

ncbi acc num :

NP_001300823.1

ncbi gb acc num :

NM_001313894.1

uniprot acc num :

P63158

ncbi mol weight :

24,894 Da

ncbi pathways :

Activated TLR4 Signalling Pathway (1367531); Activation Of DNA Fragmentation Factor Pathway (1366805); Advanced Glycosylation Endproduct Receptor Signaling Pathway (1367550); Apoptosis Pathway (1366778); Apoptosis Induced DNA Fragmentation Pathway (1366804); Apoptotic Execution Phase Pathway (1366799); Base Excision Repair Pathway (83240); Base Excision Repair Pathway (451); Cytosolic Sensors Of Pathogen-associated DNA Pathway (1367558); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (1367566)

ncbi summary :

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]

uniprot summary :

Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury. Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance (PubMed:23519706, PubMed:23446148, PubMed:23994764, PubMed:25048472). Has proangiogenic activity (PubMed:16365390). May be involved in platelet activation. Binds to phosphatidylserine and phosphatidylethanolamide. Bound to RAGE mediates signaling for neuronal outgrowth. May play a role in accumulation of expanded polyglutamine (polyQ) proteins ().

size1 :

48-Strip-Wells

price1 :

405 USD

size2 :

96-Strip-Wells

price2 :

675

size3 :

5x96-Strip-Wells

price3 :

3080

size4 :

10x96-Strip-Wells

price4 :

5805

more info or order :

MyBioSource product webpage