antibody

Rabbit anti-human Alpha-fetoprotein  (AFP) Polyclonal antibody

MBS286415

0.1 mg

245 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA, enzyme immunoassay

Antibody

Rabbit anti-human Alpha-fetoprotein  (AFP) Polyclonal antibody

[Alpha-fetoprotein  (AFP)]

[A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein]

[alpha-fetoprotein; Alpha-fetoprotein; alpha-fetoprotein; alpha fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin]

[AFP]

[AFP; AFP; AFPD; FETA; HPAFP; HPAFP]

Polyclonal

IgG

Rabbit

Human

609

The antibody is a Rabbit polyclonal antibody raised against AFP. It has been selected for its ability to recognize AFP in ELISA Kit and western blotting.

>=90% by SDS-PAGE. Rabbit Polyclonal IgG affinity purified by Protein A.

Lyophilized or Liquid

1 mg/mL

Store at -20°C for one year. Avoid repeated freeze/thaw cyles . The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C . When reconstituted in sterile pH 7.4 PBS or diluent of antibody is stable for at least two weeks at 2-8°C. Shipped at 2-8°C. Upon delivery aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.

SDS-PAGE; Western Blot (WB); ELISA (EIA)

Western Blot 1:100-500. ELIA 1:500-1000. Not yet tested in other applications. Optimal workind dilutions must be determined by the end user.

Source: Polyclonal antibody preparation. Immunogen: Recombinant human Alpha-fetoprotein  (AFP). Buffer: 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

AFP is a glycoprotein with a molecular weight of between 65,000 and 70,000 daltons including 4% of crbohydrate. During fetal development, AFP mantains high levels in the serum and drops to very low throughout the remainder of life. AFP is elevated in the malignant disesases of hepatocellular, testicular nonseminomatous origin, and ocasionally of other endotermal origin. AFP may be slightly elevated or persisted in the patients with large hepatic metastases or viral hepatitis. AFP measurement is widely accepted as tumor marker and for monitoring the therapeutic effectiveness of hepatocellular cancer and nonseminomatous testicular cancer.

4501989

NP_001125.1

NM_001134.2

P02771

Direct P53 Effectors Pathway (137939); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Glucocorticoid Receptor Regulatory Network Pathway (138014); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388)

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

0.1 mg

245 USD

0.5 mg

620

1 mg

850