antibody

Rabbit anti-human Apolipoprotein A1 (APOA1) Polyclonal antibody

MBS286368

0.1 mg

270 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA, enzyme immunoassay, immunohistochemistry - paraffin section

Antibody

Rabbit anti-human Apolipoprotein A1 (APOA1) Polyclonal antibody

[Apolipoprotein A1 (APOA1)]

[Apo-A1; ApoA-1 Milano; Proapolipoprotein A-I; Truncated apolipoprotein A-I; ProapoA-I.]

[apolipoprotein A1, partial; Apolipoprotein A-I; apolipoprotein A-I; apolipoprotein A1; Apolipoprotein A1]

[APOA1]

[APOA1; APOA1; apo(a); Apo-AI; ApoA-I; ProapoA-I]

Polyclonal

IgG

Rabbit

Human, Mouse.

67

Rabbit Polyclonal IgG, the antibody was affinity-purified by Protein A from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Liquid; 0.01M PBS (pH 7.4) with 0.5% BSA, 50% glycerol and 0.02% sodium azide.

1 mg/mL

Shipped at 2-8°C, Store it under sterile conditions at -20°C to -80°C upon receiving. **Avoid repeated freeze-thaw cycles.**. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.

Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P).

WB 1:500-2000. ELISA 1:5000-20000. IHC-P 1:100-300. Not yet tested in other applications. Optimal working dilutions must be determined by the end user.

Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human APOA1, AA range:81-130 (P02647). Predicted MW: 31 kDa

Apolipoprotein A-I the major protein component of hig h density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I was also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. ApoA-1 Milano is a naturally occurring mutant of ApoA-I, found in a family descended from a single couple of the 18th century.

4960066

AAD34604.1

ABC-family Proteins Mediated Transport Pathway (106573); ABCA Transporters In Lipid Homeostasis Pathway (477112); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Amyloids Pathway (366238); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911)

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein. [provided by RefSeq, Dec 2015]

APOA1: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Belongs to the apolipoprotein A1/A4/E family. Protein type: Cell development/differentiation; Endoplasmic reticulum; Lipid-binding; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Vesicle. Chromosomal Location of Human Ortholog: 11q23.3. Cellular Component: chylomicron; cytoplasmic vesicle; cytosol; early endosome; endocytic vesicle; endoplasmic reticulum lumen; extracellular region; extracellular space; plasma membrane. Molecular Function: apolipoprotein A-I receptor binding; apolipoprotein receptor binding; beta-amyloid binding; chemorepellent activity; cholesterol binding; cholesterol transporter activity; enzyme binding; identical protein binding; phosphatidylcholine binding; phospholipid binding; protein binding. Biological Process: cellular protein metabolic process; cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; G-protein coupled receptor protein signaling pathway; integrin-mediated signaling pathway; lipoprotein biosynthetic process; lipoprotein metabolic process; negative chemotaxis; negative regulation of cytokine secretion during immune response; negative regulation of heterotypic cell-cell adhesion; negative regulation of inflammatory response; negative regulation of interleukin-1 beta secretion; neurite regeneration; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; phospholipid efflux; phospholipid homeostasis; platelet degranulation; positive regulation of fatty acid biosynthetic process; positive regulation of hydrolase activity; positive regulation of lipoprotein lipase activity; positive regulation of Rho protein signal transduction; positive regulation of stress fiber formation; protein amino acid oxidation; protein stabilization; receptor-mediated endocytosis; regulation of Cdc42 protein signal transduction; regulation of cholesterol absorption; retinoid metabolic process; reverse cholesterol transport; transforming growth factor beta receptor signaling pathway; transmembrane transport; triacylglycerol catabolic process; vitamin transport. Disease: Amyloidosis, Familial Visceral; Hypoalphalipoproteinemia, Primary

0.1 mg

270 USD

0.5 mg

695

1 mg

925