catalog number :
MBS286271
products full name :
Rabbit anti-human Procollagen I N-Terminal Propeptide (PINP) Polyclonal antibody
products short name :
[Procollagen I N-Terminal Propeptide (PINP)]
products name syn :
[P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide]
products gene name :
[PINP]
reactivity :
Human, Mouse, Rat, Dog, Cow, Horse, Sheep.
purity :
Rabbit Polyclonal IgG, the antibody was affinity-purified by Protein A from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
form :
Liquid; 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin 300 and 50% Glycerol.
storage stability :
Storage: Shipped at 2-8°C, Store it under sterile conditions at -20°C to -80°C upon receiving. **Avoid repeated freeze-thaw cycles.**. Stability: Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.
tested application :
Western Blot (WB), ELISA (EIA), Immunohistochemistry - Frozen/Paraffin (IHC-F/P), Immunocytochemistry (ICC), Immunofluorescence (IF). Not yet tested in other applications.
app notes :
WB=1:500-2000. EIA=1:500-1000. IHC-P=1:400-800. IHC-F=1:400-800. ICC=1:100-500. IF=1:100-500. Optimal working dilutions must be determined by the end user.
image1 heading :
SDS-PAGE
other info1 :
Immunogen: KLH conjugated synthetic peptide derived from human Procollagen type I N terminal propeptide (P02452).
products description :
The PINP molecule is similar to PIIINP consisting of three distinct structural domains: Col 1 is on the aminoterminal side of the molecule, while Col 2 and Col 3 are situated on the middle of the helically structured molecule (Kühn et al. 1982). The PINP molecule has a molecular mass of 35 000 and is cleared by scavenger receptors in liver endothelial cells (Melkko et al. 1994). PINP often occurs in circulation in two forms of different molecular sizes. One is identical to the trimeric authentic antigen (intact PINP) whereas the other consists of smaller forms of PINP, resembling a single domain of the proalpha1(I) chain of PINP and is probably a degradation product of type I procollagen or I pN-collagen. Thus, an assay of intact PINP rather than total PINP appears to be more sensitive in detecting changes in the rate of type I collagen synthesis (Melkko et al. 1996, Risteli & Risteli 1999).
ncbi mol weight :
Predicted: 15 kDa