protein

Recombinant Human KRAS Protein

MBS286216

0.1 mg

370 USD

Recombinant Protein

Recombinant Human KRAS Protein

[KRAS]

[C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS3; RASK2]

[GTPase KRas isoform b; GTPase KRas; GTPase KRas; KRAS proto-oncogene, GTPase; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras]

[KRAS]

[KRAS; KRAS; NS; NS3; CFC2; RALD; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2; KRAS2; RASK2]

RASK_HUMAN

E. coli AA 1-188 (P01116).

188

VPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM
MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDS
YRKQVVIDGETCLLDILDTAGHEEYSAMRDQYMRTGEGF
LCVFAINNTKSFEDIHHYREQIKRVKDSED

> 90% as determined by SDS-PAGE.

58 mM Na2HPO4, 17 mM NaH2PO4, 68 mM NaCl, pH 7.4 added with 300 mM Imidazole and 15% glycerol.

0.9 mg/mL

Store it under sterile condition s at-20°C upon receiving. Recommend to aliquot theprotein into smaller quantities for optimal storage.**Avoid repeated freeze-thaw cycles.**. Stability: The recombinant protein is stable for up to 6 months from date of receipt at -80°C

Western Blot (WB), ELISA (EIA), SDS-PAGE, Antigen

SDS-PAGE

Source: Human. Protein Residues: with N-terminal 6*His. Predicted MW: 27 kDa. Observed MW: 27 kDa. Usage: KRAS Protein-Centrifuge the standard vial at 6000-10000 rpm for 30s.

Like other members of the Ras family, the KRAS protein is a GTPase and is an early player in many signal transduction pathways. KRAS is usually tethered to cell membranes because of the presence of an isoprenyl group on its C-terminus. When mutated, KRAS is a oncogene. The protein product of the normal KRAS gene performs an essential function in normal tissue signaling, and the mutation of a KRAS gene is an essential step in the development of many cancers. KRAS acts as a molecular on/off switch, once it is turned on it recruits and activates proteins necessary for the propagation of growth factor and other receptors' signal, such as c-Raf and PI 3-kinase. KRAS binds to GTP in the active state and possesses an intrinsic enzymatic activity which cleaves the terminal phosphate of the nucleotide converting it to GDP.

15718761

NP_004976.2

NM_004985.4

P01116

22kDa

AGE-RAGE Signaling Pathway In Diabetic Complications (1319988); AGE-RAGE Signaling Pathway In Diabetic Complications (1319775); ARMS-mediated Activation Pathway (1269471); Activation Of RAS In B Cells Pathway (1269187); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adaptive Immune System Pathway (1269171); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Aldosterone-regulated Sodium Reabsorption Pathway (130626)

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (PubMed:24623306).

0.1 mg

370 USD

0.5 mg

1045

1 mg

1455