catalog number :
MBS285996
products type :
ELISA Kit
products full name :
Human 27-hydroxycholesterol ELISA Kit
products short name :
[27-hydroxycholesterol]
products gene name :
[27HC]
specificity :
This assay has high sensitivity and excellent specificity for detection of Human 27-hydroxycholesterol. No significant cross-reactivity or interference between Human 27-hydroxycholesterol and analogues was observed.
storage stability :
Store at 2-8 degree C.
other info1 :
Samples: serum, plasma and other biological fluids. Assay Type: Quantitative Competitive. Detection Range: 1.56 pg/mL - 100 pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: For the quantitative detection of Human 27-hydroxycholesterol concentration in serum, plasma and other biological fluids. Principle of the Assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 27-hydroxycholesterol. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)- conjugated 27-hydroxycholesterol and incubated. The competitive inhibition reaction is launched between with HRP labeled 27-hydroxycholesterol and unlabeled 27-hydroxycholesterol with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of 27-hydroxycholesterol in the sample. The color development is stopped and the intensity of the color is measured.
size4 :
10x96-Strip-Wells