catalog number :
MBS285887
products type :
ELISA Kit
products full name :
Rat Interleukin 23 (IL-23) ELISA Kit
products short name :
[Interleukin 23]
products gene name :
[IL-23]
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat IL-23. No significant cross-reactivity or interference between Rat IL-23 and analogues was observed.
storage stability :
Store at 2-8 degree C.
other info1 :
Samples: Serum, Plasma, Other biological fluids. Assay Type: Sandwich
other info2 :
Sample Volume: 1-200 uL. Precision: Intra-assay Precision (Precision within an assay). Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays). Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Intra-Assay: CV. Inter-Assay: CV. Detection Wavelength: 450 nm
products description :
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IL-23 in samples. An antibody specific for IL-23 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL-23 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-23 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-23 bound in the initial step. The color development is stopped and the intensity of the color is measured.
size4 :
10x96-Strip-Wells