Human Semaphorin 3A (Sema 3A) ELISA Kit | MyBioSource MBS285829 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Semaphorin 3A (Sema 3A) ELISA Kit

catalog :

MBS285829

quantity :

48-Strip-Wells

price :

405 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS285829

products type :

ELISA Kit

products full name :

Human Semaphorin 3A (Sema 3A) ELISA Kit

products short name :

[Semaphorin 3A]

products name syn :

[Sema 3A; Hsema-I; Hsema-III; MGC133243; SEMA1; SEMAD; SEMAIII; SEMAL; SemD; coll-1; OTTHUMP00000208884 collapsin 1 semaphorin 3A semaphorin D semaphorin III semaphorin L semaphorin-like]

other names :

[semaphorin-3A; Semaphorin-3A; semaphorin-3A; semaphorin 3A; Semaphorin III; Sema III]

products gene name :

[SEMA3A]

other gene names :

[SEMA3A; SEMA3A; HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III; SEMAD; Sema III]

reactivity :

Human

sequence length :

771

specificity :

This assay has high sensitivity and excellent specificity for detection of Human S100A8/A9. No significant cross-reactivity or interference between Human S100A8/A9 and analogues was observed.

storage stability :

Store at 2-8 degree C.

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 1.56 ng/mL-100 ng/mL. Sensitivity: 0.52 ng/mL (mean of 6 independent assays).

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate S100A8/A9 in Human serum, plasma. An antibody specific for S100A8/A9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100A8/A9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A8/A9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A8/A9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

5174673

ncbi acc num :

NP_006071.1

ncbi gb acc num :

NM_006080.2

uniprot acc num :

Q14563

ncbi mol weight :

88,889 Da

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); CRMPs In Sema3A Signaling Pathway (119525); Developmental Biology Pathway (477129); SEMA3A-Plexin Repulsion Signaling By Inhibiting Integrin Adhesion Pathway (119523); Sema3A PAK Dependent Axon Repulsion Pathway (119524); Semaphorin Interactions Pathway (119519)

ncbi summary :

This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq, Jul 2008]

uniprot summary :

SEMA3A: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. Belongs to the semaphorin family. Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7q21.11. Cellular Component: axon; dendrite; extracellular region; extracellular space. Molecular Function: chemorepellent activity; semaphorin receptor binding. Biological Process: apoptosis; axon extension involved in axon guidance; axon guidance; axonal fasciculation; branchiomotor neuron axon guidance; dendrite morphogenesis; facial nerve structural organization; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; negative chemotaxis; negative regulation of axon extension involved in axon guidance; nerve development; neuron migration; olfactory bulb development; regulation of axon extension involved in axon guidance; regulation of heart rate; sensory system development; sympathetic nervous system development; trigeminal nerve structural organization. Disease: Hypogonadotropic Hypogonadism 16 With Or Without Anosmia

size1 :

48-Strip-Wells

price1 :

405 USD

size2 :

96-Strip-Wells

price2 :

675

size3 :

5x96-Strip-Wells

price3 :

3080

size4 :

10x96-Strip-Wells

price4 :

5805

more info or order :

MyBioSource product webpage