ELISA/assay

Human Inhibin B (INHB) ELISA Kit

MBS285641

48-Strip-Wells

405 USD

ELISA Kit

Human Inhibin B (INHB) ELISA Kit

[Inhibin B]

[inhibin beta B chain preproprotein; Inhibin beta B chain; inhibin beta B chain; inhibin beta B; Activin beta-B chain]

[INHB]

[INHBB; INHBB]

INHBB_HUMAN

Human

407

This assay has high sensitivity and excellent specificity for detection of Human INHB. No significant cross-reactivity or interference between Human INHB and analogues was observed.

Store at 2-8 degree C.

Samples: Serum, Plasma, Other biological fluids. Assay Type: Sandwich

Sample Volume: 1-200 uL. Precision: Intra-assay Precision (Precision within an assay). Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays). Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Intra-Assay: CV. Inter-Assay: CV. Detection Wavelength: 450 nm

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate INHB in samples. An antibody specific for INHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyINHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for INHB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of INHB bound in the initial step. The color development is stopped and the intensity of the color is measured.

154813204

NP_002184.2

NM_002193.2

45,122 Da

AGE/RAGE Pathway (698754); Activin Signaling Pathway (1084757); Activin Signaling Pathway (1108220); Antagonism Of Activin By Follistatin Pathway (1269622); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Glycoprotein Hormones Pathway (1268754); Metabolism Of Proteins Pathway (1268677); Peptide Hormone Biosynthesis Pathway (1268753); Peptide Hormone Metabolism Pathway (1268746)

The inhibin beta B subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumour-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta B subunit forms a homodimer, activin B, and also joins with the beta A subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. [provided by RefSeq, Jul 2008]

INHBB: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family. Protein type: Secreted, signal peptide; Cell development/differentiation; Cytokine; Secreted. Chromosomal Location of Human Ortholog: 2cen-q13. Cellular Component: extracellular region; extracellular space; perinuclear region of cytoplasm. Molecular Function: cytokine activity; growth factor activity; hormone activity; host cell surface receptor binding; protein binding; protein homodimerization activity; transforming growth factor beta receptor binding. Biological Process: activin receptor signaling pathway; cell development; cell differentiation; cellular response to insulin stimulus; cellular response to starvation; defense response; fat cell differentiation; growth; negative regulation of follicle-stimulating hormone secretion; negative regulation of hepatocyte growth factor biosynthetic process; negative regulation of insulin secretion; oocyte development; ovarian follicle development; positive regulation of follicle-stimulating hormone secretion; regulation of apoptosis; regulation of MAPKKK cascade; response to mechanical stimulus

48-Strip-Wells

405 USD

96-Strip-Wells

675

5x96-Strip-Wells

3080

10x96-Strip-Wells

5805