Human Renin (REN) ELISA Kit | MyBioSource MBS285124 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Renin (REN) ELISA Kit

catalog :

MBS285124

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS285124

products type :

ELISA Kit

products full name :

Human Renin (REN) ELISA Kit

products short name :

[Renin]

products name syn :

[LJ10761; HNFJ2; angiotensin-forming enzyme angiotensinogenase renin precursor; renal]

other names :

[renin preproprotein; Renin; renin; renin; Angiotensinogenase]

products gene name :

[REN]

other gene names :

[REN; REN; HNFJ2]

reactivity :

Human

sequence length :

406

specificity :

This assay has high sensitivity and excellent specificity for detection of Human REN. No significant cross-reactivity or interference between Human REN and analogues was observed.

storage stability :

Store at 2-8 degree C.

other info1 :

Samples: Serum, Plasma, Other biological fluids. Assay Type: Sandwich

other info2 :

Sample Volume: 1-200 uL. Precision: Intra-assay Precision (Precision within an assay). Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays). Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Intra-Assay: CV. Inter-Assay: CV. Detection Wavelength: 450 nm

products description :

Renin is a member of the aspartyl proteinase family produced largely in part by the juxtaglomerular cells in the kidney.Renin differs from the other members of this class by having a pH optimum near neutral pH region with native substrates instead of 2.0 to 3.4 This more neutral pH optimum allows it to be functional in the plasma. Renin also has very high selectivity for substrate due to quite long peptide recognition on either side of the peptide bond undergoing cleavage. An octapeptide substrate was the minimum length to be cleaved by renin. Renin plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen, which is the only known physiological substrate of renin. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate REN in samples. An antibody specific for REN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyREN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REN bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4506475

ncbi acc num :

NP_000528.1

ncbi gb acc num :

NM_000537.3

uniprot acc num :

P00797

ncbi mol weight :

44,726 Da

ncbi pathways :

ACE Inhibitor Pathway (198763); Metabolism Of Angiotensinogen To Angiotensins Pathway (685555); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Renin-angiotensin System Pathway (83075); Renin-angiotensin System Pathway (486)

ncbi summary :

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq, Jul 2008]

uniprot summary :

REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.23.15; Protease; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q32.1. Cellular Component: apical part of cell; cytoplasm; extracellular region; extracellular space; plasma membrane. Molecular Function: aspartic-type endopeptidase activity; insulin-like growth factor receptor binding; peptidase activity; protein binding; receptor binding. Biological Process: angiotensin maturation; beta-amyloid metabolic process; cell maturation; drinking behavior; hormone-mediated signaling pathway; kidney development; male gonad development; mesonephros development; protein catabolic process; proteolysis; regulation of blood pressure; regulation of MAPK cascade; renin-angiotensin regulation of aldosterone production; response to cAMP; response to cGMP; response to lipopolysaccharide. Disease: Hyperuricemic Nephropathy, Familial Juvenile, 2; Renal Tubular Dysgenesis

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

745

size3 :

5x96-Strip-Wells

price3 :

3405

size4 :

10x96-Strip-Wells

price4 :

6425

more info or order :

MyBioSource product webpage