This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat Osteopontin (OPN) ELISA Kit
catalog :
MBS285050
quantity :
48-Strip-Wells
price :
440 USD
product information
catalog number :
MBS285050
products type :
ELISA Kit
products full name :
Rat Osteopontin (OPN) ELISA Kit
products short name :
[Osteopontin]
products name syn :
[BNSP; BSPI; ETA-1; MGC110940; OPN; SPP1/CALPHA1 fusion bone sialoprotein I early T-lymphocyte activation 1 osteopontin osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein s]
other names :
[osteopontin; Osteopontin; osteopontin; Bone sialoprotein 1; OC-1; Secreted phosphoprotein 1; SPP-1]
products gene name :
[SPP1]
other gene names :
[SPP1; SPP1; OPN; SPP-1]
reactivity :
Rat
sequence length :
300
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat SPP1. No significant cross-reactivity or interference between Rat SPP1 and analogues was observed.
storage stability :
Store at 2-8 degree C.
other info1 :
Samples: Serum, Plasma, Other biological fluids. Assay Type: Sandwich
other info2 :
Sample Volume: 1-200 uL. Precision: Intra-assay Precision (Precision within an assay). Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays). Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Intra-Assay: CV. Inter-Assay: CV. Detection Wavelength: 450 nm
products description :
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate SPP1 in samples. An antibody specific for SPP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
126723455
ncbi acc num :
NP_001075663.1
ncbi gb acc num :
NM_001082194.1
uniprot acc num :
P31097
ncbi mol weight :
35,172 Da
ncbi pathways :
ECM-receptor Interaction Pathway (1072871); ECM-receptor Interaction Pathway (479); Focal Adhesion Pathway (1072873); Focal Adhesion Pathway (478); PI3K-Akt Signaling Pathway (1072856); PI3K-Akt Signaling Pathway (692979); Toll-like Receptor Signaling Pathway (1072988); Toll-like Receptor Signaling Pathway (487)
uniprot summary :
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
size1 :
48-Strip-Wells
price1 :
440 USD
size2 :
96-Strip-Wells
price2 :
745
size3 :
5x96-Strip-Wells
price3 :
3405
size4 :
10x96-Strip-Wells
price4 :
6425
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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