Human Osteopontin (OPN) ELISA Kit | MyBioSource MBS284664 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Osteopontin (OPN) ELISA Kit

catalog :

MBS284664

quantity :

48-Strip-Wells

price :

495 USD

product information

catalog number :

MBS284664

products type :

ELISA Kit

products full name :

Human Osteopontin (OPN) ELISA Kit

products short name :

[Osteopontin]

products name syn :

[BNSP; BSPI; ETA-1; MGC110940; OPN; SPP1/CALPHA1 fusion bone sialoprotein I early T-lymphocyte activation 1 osteopontin osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein s]

other names :

[osteopontin isoform OPN-b; Osteopontin; osteopontin; secreted phosphoprotein 1; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin]

products gene name :

[SPP1]

other gene names :

[SPP1; SPP1; OPN; BNSP; BSPI; ETA-1; BNSP; OPN; SPP-1]

reactivity :

Human

sequence length :

300

specificity :

This assay has high sensitivity and excellent specificity for detection of Human SPP1. No significant cross-reactivity or interference between Human SPP1 and analogues was observed.

storage stability :

Store at 2-8 degree C.

other info1 :

Samples: Serum, Plasma, Other biological fluids. Assay Type: Sandwich

other info2 :

Sample Volume: 1-200 uL. Precision: Intra-assay Precision (Precision within an assay). Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays). Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Intra-Assay: CV. Inter-Assay: CV. Detection Wavelength: 450 nm

products description :

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate SPP1 in samples. An antibody specific for SPP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4759166

ncbi acc num :

NP_000573.1

ncbi gb acc num :

NM_000582.2

uniprot acc num :

P10451

ncbi mol weight :

33,843 Da

ncbi pathways :

BDNF Signaling Pathway (712093); Direct P53 Effectors Pathway (137939); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Endochondral Ossification Pathway (198812); FGF Signaling Pathway (137989); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Integrin Cell Surface Interactions Pathway (106110)

ncbi summary :

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

uniprot summary :

osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q22.1. Cellular Component: cell projection; endoplasmic reticulum lumen; extracellular exosome; extracellular region; extracellular space; Golgi apparatus; perinuclear region of cytoplasm. Molecular Function: cytokine activity; extracellular matrix binding; protein binding. Biological Process: androgen catabolic process; biomineral formation; cell adhesion; cellular protein metabolic process; cellular response to testosterone stimulus; decidualization; embryo implantation; extracellular matrix disassembly; extracellular matrix organization; inflammatory response; negative regulation of collateral sprouting of intact axon in response to injury; osteoblast differentiation; positive regulation of bone resorption; positive regulation of estradiol secretion; positive regulation of transcription, DNA-templated; post-translational protein modification; regulation of receptor activity; response to steroid hormone; response to vitamin D

size1 :

48-Strip-Wells

price1 :

495 USD

size2 :

96-Strip-Wells

price2 :

860

size3 :

5x96-Strip-Wells

price3 :

3960

size4 :

10x96-Strip-Wells

price4 :

7425