ELISA/assay

Human Interleukin-15 receptor subunit alpha (IL15RA) ELISA Kit

MBS284419

48-Strip-Wells

485 USD

ELISA Kit

Human Interleukin-15 receptor subunit alpha (IL15RA) ELISA Kit

Interleukin-15

RP11-536K7.2; MGC104179;

interleukin-15

IL15RA

Human

162

This assay has high sensitivity and excellent specificity for detection of Human IL15RA. No significant cross-reactivity or interference between Human IL15RA and analogues was observed.

Store at 2-8 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25 pg/mL - 2000 pg/mL. The standard curve concentrations used for the ELISA's were 2000 pg/mL, 1000 pg/mL, 500 pg/mL, 250 pg/mL, 125 pg/mL, 62.5 pg/mL, 31.25 pg/mL, 0 pg/mL. Sensitivity: 15.6 pg/mL (mean of 6 independent assays).

Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: For the quantitative detection of Human Interleukin-15 receptor subunit alpha (IL15RA) concentration in serum, plasma and other biological fluids. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IL15RA in Human serum, plasma. An antibody specific for IL15RA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL15RA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL15RA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL15RA bound in the initial step. The color development is stopped and the intensity of the color is measured.

47523834

NP_999555.1

NM_214390.1

Cytokine-cytokine Receptor Interaction Pathway (84463); Cytokine-cytokine Receptor Interaction Pathway (460); HTLV-I Infection Pathway (373897); HTLV-I Infection Pathway (373889); Herpes Simplex Infection Pathway (377870); Herpes Simplex Infection Pathway (377865); Intestinal Immune Network For IgA Production Pathway (128755); Intestinal Immune Network For IgA Production Pathway (128670); Jak-STAT Signaling Pathway (84489); Jak-STAT Signaling Pathway (488)

48-Strip-Wells

485 USD

96-Strip-Wells

820