ELISA/assay

Human Mucin-5 subtype B (MUC5B) ELISA Kit

MBS284008

48-Strip-Wells

405 USD

ELISA Kit

Human Mucin-5 subtype B (MUC5B) ELISA Kit

[Mucin-5 subtype B]

[MG1; MUC5; MUC9; cervical mucin MUC5B high molecular weight mucin mucin 5; subtype B; tracheobronchial mucin MUC5B mucin; salivary (high molecular weight)]

[mucin-5AC; Mucin-5AC; mucin-5AC; mucin 5AC, oligomeric mucus/gel-forming; Gastric mucin]

[MUC5B]

[MUC5AC; MUC5AC; TBM; leB; MUC5; mucin; TBM]

Human

This assay has high sensitivity and excellent specificity for detection of Human MUC5B. No significant cross-reactivity or interference between Human MUC5B and analogues was observed.

Store at 2-8 degree C.

Samples: Serum, Plasma, Other biological fluids. Assay Type: Sandwich

Sample Volume: 1-200 uL. Precision: Intra-assay Precision (Precision within an assay). Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays). Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Intra-Assay: CV. Inter-Assay: CV. Detection Wavelength: 450 nm

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MUC5B in samples. An antibody specific for MUC5B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMUC5B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC5B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC5B bound in the initial step. The color development is stopped and the intensity of the color is measured.

748983076

NP_001291288.1

NM_001304359.1

P98088

585,570 Da

Defective C1GALT1C1 Causes Tn Polyagglutination Syndrome (TNPS) Pathway (1383050); Defective GALNT12 Causes Colorectal Cancer 1 (CRCS1) Pathway (1383052); Defective GALNT3 Causes Familial Hyperphosphatemic Tumoral Calcinosis (HFTC) Pathway (1383051); Disease Pathway (1268854); Diseases Associated With O-glycosylation Of Proteins Pathway (1383049); Diseases Of Glycosylation Pathway (1269010); Metabolism Of Proteins Pathway (1268677); O-linked Glycosylation Pathway (1268734); O-linked Glycosylation Of Mucins Pathway (1268736); Post-translational Protein Modification Pathway (1268701)

MUC5AC iso1: . Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: cytoplasm; extracellular space; Golgi lumen; plasma membrane. Molecular Function: extracellular matrix structural constituent. Biological Process: O-glycan processing; phosphoinositide-mediated signaling; stimulatory C-type lectin receptor signaling pathway

48-Strip-Wells

405 USD

96-Strip-Wells

675

5x96-Strip-Wells

3080

10x96-Strip-Wells

5805