ELISA/assay

Human Ghrelin (GHRL) ELISA Kit

MBS283919

48-Strip-Wells

440 USD

ELISA Kit

Human Ghrelin (GHRL) ELISA Kit

[Ghrelin]

[MTLRP; obestatin; OTTHUMP00000207794 OTTHUMP00000207795 OTTHUMP00000207796 OTTHUMP00000207798 ghrelin; growth hormone secretagogue receptor ligand ghrelin/obestatin ghrelin/obestatin preprohormone m]

[appetite-regulating hormone isoform 2 preproprotein; Appetite-regulating hormone; appetite-regulating hormone; ghrelin and obestatin prepropeptide; Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related peptide; Protein M46]

[GHRL]

[GHRL; GHRL; MTLRP; MTLRP; Ghrelin]

Human

117

This assay has high sensitivity and excellent specificity for detection of Human GHRL. No significant cross-reactivity or interference between Human GHRL and analogues was observed.

Store at 2-8 degree C.

Samples: Serum, Plasma, Other biological fluids. Assay Type: Sandwich

Sample Volume: 1-200 uL. Precision: Intra-assay Precision (Precision within an assay). Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays). Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Intra-Assay: CV. Inter-Assay: CV. Detection Wavelength: 450 nm

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GHRL in samples. An antibody specific for GHRL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGHRL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GHRL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GHRL bound in the initial step. The color development is stopped and the intensity of the color is measured.

201860279

NP_001128413.1

NM_001134941.2

Q9UBU3

9,972 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Metabolic Disorders Of Biological Oxidation Enzymes Pathway (1127637); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603)

This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014]

ghrelin: a hormone that binds to the growth hormone secretagogue receptor type 1 (GHSR). Secreted by the stomach. Protein type: Apoptosis; Cell cycle regulation; Cell development/differentiation; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3p25.3. Cellular Component: axon; endoplasmic reticulum lumen; extracellular region; extracellular space. Molecular Function: G-protein coupled receptor binding; ghrelin receptor binding; growth hormone-releasing hormone activity; protein binding; protein tyrosine kinase activator activity. Biological Process: actin polymerization or depolymerization; activation of MAPK activity; adult feeding behavior; cartilage development; cortisol secretion; decidualization; dendrite development; elevation of cytosolic calcium ion concentration; excitatory postsynaptic potential; G-protein coupled receptor signaling pathway; gastric acid secretion; glucose metabolic process; growth hormone secretion; hormone-mediated signaling pathway; negative regulation of angiogenesis; negative regulation of apoptosis; negative regulation of circadian sleep/wake cycle, REM sleep; negative regulation of endothelial cell proliferation; negative regulation of inflammatory response; negative regulation of insulin secretion; negative regulation of interleukin-1 beta production; negative regulation of interleukin-6 biosynthetic process; negative regulation of locomotion; negative regulation of tumor necrosis factor biosynthetic process; positive regulation of appetite; positive regulation of circadian sleep/wake cycle, non-REM sleep; positive regulation of corticotropin secretion; positive regulation of cortisol secretion; positive regulation of growth hormone secretion; positive regulation of growth rate; positive regulation of insulin secretion; positive regulation of multicellular organism growth; positive regulation of synaptogenesis; regulation of cell proliferation; regulation of response to food; regulation of transmission of nerve impulse; response to electrical stimulus; response to estrogen; response to hormone. Disease: Obesity

48-Strip-Wells

440 USD

96-Strip-Wells

745

5x96-Strip-Wells

3405

10x96-Strip-Wells

6425