catalog number :
MBS283658
products type :
Recombinant Protein
products full name :
Recombinant Human Cytokeratin Fragment Antigen 21-1 (CYFRA21-1)
products short name :
[Cytokeratin Fragment Antigen 21-1]
products name syn :
[K1C19_HUMAN;Keratin,typeIcytoskeletal19;Cytokeratin-19;CK-19;Keratin-19;K19]
products gene name :
[CYFRA21-1]
sequence :
EKLTMQNLNDRLASYLDKVRALEAANGELEVKIRDWYQK
QGPGPSRDYSHYYTTIQDLRDKILGATIENSRIVLQIDN
ARLAADDFRTKFETEQALRMSVEADINGLRRVLDELTLA
RTDLEMQIEGLKEELAYLKKNHEEEISTLRGQVGGQVSV
EVDSAP
purity :
>95 % as determined by SDS-PAGE
form :
58 mM Na 2 HPO 4 , 17 mM NaH 2 PO 4 , 68 mM NaCl, 300 mM Imidazole, pH 8.0,Trehalose (5-8%) and mannitol (5-8%) protectants were added before lyophilization
concentration :
0.8 mg/mL
storage stability :
Store it under sterile conditions at -20°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles. The recombinant protein is stable for up to 6 months from date of receipt at -80°C.
tested application :
Western Blot (WB), ELISA (EIA)
app notes :
Western blotting, ELISA, Albumin-bound fluorescence was used in serum of patients with chronic renal failure.
other info1 :
Predicted MW: 62 kDa. Observed MW: 62 kDa. Usage: CYFRA21-1 Protein - Centrifuge the standard vial at 6000-10000rpm for 30s.
other info2 :
Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method. Storage Buffer: The protein solution is in 0.05M phosphate buffer containing 0.15M NaCl and 0.09% NaN3 pH 7.5. Filtered through a 0.4uM membrane. Protein Residues: with N-terminal GST.
products description :
Cytokeratin19 Fragment Antigen 21-1(CYFRA21-1) belongs to a family of 20 related polypeptides that are constituents of the intermediate filaments of epithelial cells. Of these CK19 is the most frequently expressed cytokeratin endometrial cancer. CYFRA21-1 is a common tumor marker of lung cancer,non-small-cell cancer, small cell carcinoma and squamous cell carcinoma. For breast cancer, CYFRA21-1 may be comparable to the sensitivity and specificity of CA153. Measured jointly with any of the tumor markers as CEA, SCCA, NSE, can increase the sensitivity. Other as bladder cancer, nasopharyngeal cancer, ovarian cancer, gastrointestinal cancer, and also have certain positive rate. CYFRA21-1 in hepatitis, pancreatitis, pneumonia, benign prostatic hyperplasia may also be increased.