Human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (HCN2) ELISA Kit | MyBioSource MBS283560 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (HCN2) ELISA Kit

catalog :

MBS283560

quantity :

48-Strip-Wells

price :

485 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS283560

products type :

ELISA Kit

products full name :

Human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (HCN2) ELISA Kit

products short name :

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

other names :

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2; potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2; brain cyclic nucleotide gated channel 2; brain cyclic nucleotide-gated channel 2; hyperpolarization activated cyclic nucleotide-gated potassium channel 2; Brain cyclic nucleotide-gated channel 2; BCNG-2

products gene name :

HCN2

other gene names :

HCN2; HCN2; BCNG2; HAC-1; BCNG-2; BCNG2; BCNG-2

uniprot entry name :

HCN2_HUMAN

reactivity :

Human

sequence length :

889

specificity :

This assay has high sensitivity and excellent specificity for detection of Human HCN2. No significant cross-reactivity or interference between Human HCN2 and analogues was observed.

storage stability :

Store at 2-8 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.31 ng/mL - 20 ng/mL. Sensitivity: The limit of detection of Human HCN2 defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 0.1 ng/mL (mean of 6 independent assays).

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%. Intended Uses: For the quantitative detection of Human Potassium/sodium hyperpolarization -activated cyclic nucleotide-gated channel 2 (HCN2) concentration in serum, plasma and other biological fluids.

products description :

Principle of the assay: This assay employs a two-site sandwich ELISA to quantitative HCN2 in Human serum, plasma. An antibody specific for HCN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HCN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for HCN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HCN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

108935843

ncbi acc num :

Q9UL51.3

ncbi mol weight :

96,950 Da

ncbi pathways :

HCN Channels Pathway (366223); Neuronal System Pathway (106513); Potassium Channels Pathway (366221); CAMP Signaling Pathway (1017634); CAMP Signaling Pathway (1019520)

ncbi summary :

Hyperpolarization-activated cation channels of the HCN gene family, such as HCN2, contribute to spontaneous rhythmic activity in both heart and brain.[supplied by OMIM, Jul 2010]

uniprot summary :

HCN2: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages. Belongs to the potassium channel HCN family. Protein type: Channel, cation; Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: voltage-gated potassium channel complex; integral to plasma membrane; plasma membrane. Molecular Function: identical protein binding; voltage-gated potassium channel activity; intracellular cAMP activated cation channel activity; voltage-gated sodium channel activity; cAMP binding. Biological Process: synaptic transmission; regulation of membrane potential; cell-cell signaling

size1 :

48-Strip-Wells

price1 :

485 USD

size2 :

96-Strip-Wells

price2 :

820

more info or order :

MyBioSource product webpage