catalog number :
MBS283380
products type :
ELISA Kit
products full name :
Fish Interleukin 1beta (IL-1beta) ELISA Kit
products short name :
[Interleukin 1beta]
products gene name :
[IL-1beta]
specificity :
This assay has high sensitivity and excellent specificity for detection of Fish IL1B. No significant cross-reactivity or interference between Fish IL1B and analogues was observed.
storage stability :
Store at 2-8 degree C.
other info1 :
Samples: Serum, Plasma, Other biological fluids. Assay Type: Sandwich
other info2 :
Sample Volume: 1-200 uL. Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.CV (%) = SD/meanX100Intra-Assay: CV<8% Inter-Assay: CV<12%. Detection Wavelength: 450 nm
products description :
Interleukin-1 (IL-1) is one of the first cytokines ever described. Its initial discovery was as a factor that could induce fever, control lymphocytes, increase the number of bone marrow cells and cause degeneration of bone joints. At this time, IL-1 was known under several other names including endogenous pyrogen, lymphocyte activating factor, haemopoetin-1 and mononuclear cell factor, amongst others.It was around 1984-1985 when scientists confirmed that IL-1 was actually composed of two distinct proteins, now called IL-1. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IL1B in samples. An antibody specific for IL1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL1B bound in the initial step. The color development is stopped and the intensity of the color is measured.
size4 :
10x96-Strip-Wells