ELISA/assay

Goat C-reactive protein (CRP) ELISA Kit

MBS283288

48-Strip-Wells

510 USD

ELISA Kit

Goat C-reactive protein (CRP) ELISA Kit

C-reactive protein

C-reactive protein; C-reactive protein; C-reactive protein; pentraxin 1; C-reactive protein, pentraxin-related

CRP

CRP; CRP; PTX1; PTX1

CRP_HUMAN

Goat

224

This assay has high sensitivity and excellent specificity for detection of Goat CRP. No significant cross-reactivity or interference between Goat CRP and analogues was observed.

Store at 2-8 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Competitive. Detection Range: 150 ng/mL - 6000 ng/mL.The standard curve concentrations used for the ELISA's were 6000 ng/mL, 3000 ng/mL, 1500 ng/mL, 750 ng/mL, 300 ng/mL, 150 ng/mL. Sensitivity: The limit of detection of Goat CRP defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 58 ng/mL (mean of 6 independent assays).

Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<10%. Intended Uses: For the quantitative detection of Goat C-reactive protein (CRP) concentration in serum, plasma and other biological fluids.

Principle of the assay: This assay employs the competitive enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to CRP. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated antibody preparation specific for CRP and incubated. Then substrate solution A and B are added to each well. Only those wells that contain CRP, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CRP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

55770842

NP_000558.2

NM_000567.2

10,415 Da

Classical Antibody-mediated Complement Activation Pathway (106409); Complement Cascade Pathway (106405); Creation Of C4 And C2 Activators Pathway (106407); IL6-mediated Signaling Events Pathway (137932); Immune System Pathway (106386); Initial Triggering Of Complement Pathway (106406); Innate Immune System Pathway (106387); Selenium Pathway (198825)

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq, Sep 2009]

CRP: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Belongs to the pentaxin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q23.2. Cellular Component: extracellular space; growth cone; extracellular region; filopodium. Molecular Function: choline binding; protein binding; protein homodimerization activity; low-density lipoprotein receptor binding; complement component C1q binding; low-density lipoprotein binding; cholesterol binding; virion binding; calcium ion binding. Biological Process: protein polymerization; defense response to Gram-positive bacterium; response to ethanol; wound healing; negative regulation of vasodilation; positive regulation of superoxide release; response to lead ion; response to hypoxia; acute-phase response; opsonization; inflammatory response; complement activation, classical pathway; aging

48-Strip-Wells

510 USD

96-Strip-Wells

865