ELISA/assay

Human Nerve growth factor (NGF) ELISA Kit

MBS283264

48-Strip-Wells

445 USD

ELISA Kit

Human Nerve growth factor (NGF) ELISA Kit

Nerve growth factor

nerve growth factor; Nerve growth factor; nerve growth factor; zNGF; nerve growth factor (beta polypeptide)

NGF

ngf; ngf; ngfb; ns:zft386; xx:zft386; zgc:109730; ngfb; NGF; zNGF

NGF_DANRE

Human

194

This assay has high sensitivity and excellent specificity for detection of Human NGF. No significant cross-reactivity or interference between Human NGF and analogues was observed.

Store at 2-8 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156 ng/mL - 10 ng/mL. Sensitivity: The limit of detection of Human NGF defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 0.078 ng/mL (mean of 6 independent assays).

Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%. Intended Uses: For the quantitative detection of Human Putative potassium channel regulatory protein (NGF) concentration in serum, plasma and other biological fluids.

Principle of the assay: This assay employs a two-site sandwich ELISA to quantitative NGF in Human serum, plasma. An antibody specific for NGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for NGF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

42409517

NP_954680.1

NM_199210.1

21,903 Da

ARMS-mediated Activation Pathway (1003605); Activation Of TRKA Receptors Pathway (1003596); Apoptosis Pathway (83637); Apoptosis Pathway (470); Axonal Growth Stimulation Pathway (1003592); Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (1003583); Ceramide Signalling Pathway (1003590); Frs2-mediated Activation Pathway (1003604); MAPK Signaling Pathway (83627); MAPK Signaling Pathway (456)

48-Strip-Wells

445 USD

96-Strip-Wells

740