ELISA/assay

Human Slit homolog 2 protein (SLIT2) ELISA Kit

MBS283223

48-Strip-Wells

405 USD

ELISA Kit

Human Slit homolog 2 protein (SLIT2) ELISA Kit

[Slit homolog 2 protein]

[slit homolog 2 protein isoform 2; Slit homolog 2 protein; slit homolog 2 protein; slit guidance ligand 2]

[SLIT2]

[SLIT2; SLIT2; SLIL3; Slit-2; SLIL3; Slit-2]

Human

This assay has high sensitivity and excellent specificity for detection of Human SLIT2. No significant cross-reactivity or interference between Human SLIT2 and analogues was observed.

Store at 2-8 degree C.

Samples: Serum, Plasma, Other biological fluids. Assay Type: Sandwich

Sample Volume: 1-200 uL. Precision: Intra-assay Precision (Precision within an assay). Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays). Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Intra-Assay: CV. Inter-Assay: CV. Detection Wavelength: 450 nm

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate SLIT2 in samples. An antibody specific for SLIT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLIT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLIT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLIT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

574281850

NP_001276064.1

NM_001289135.2

O94813

168,893 Da

Activation Of Rac Pathway (1270321); Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (1270303); Developmental Biology Pathway (1270302); Glypican 1 Network Pathway (138010); Inactivation Of Cdc42 And Rac Pathway (1270320); Netrin-1 Signaling Pathway (1270314); Regulation Of Commissural Axon Pathfinding By Slit And Robo Pathway (1270319); Role Of Abl In Robo-Slit Signaling Pathway (1270322)

This gene encodes a member of the slit family of secreted glycoproteins, which are ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. Members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Proteolytic processing of this protein gives rise to an N-terminal fragment that contains the four leucine-rich repeats and five epidermal growth factor repeats and a C-terminal fragment that contains four epidermal growth factor repeats and the cysteine knot. Both full length and cleaved proteins are secreted extracellularly and can function in axon repulsion as well as other specific processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

SLIT2: Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth- stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post- crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4p15.31. Cellular Component: cytoplasm; extracellular region; extracellular space; membrane; plasma membrane. Molecular Function: calcium ion binding; GTPase inhibitor activity; heparin binding; identical protein binding; laminin-1 binding; protein binding; protein homodimerization activity; proteoglycan binding; Roundabout binding. Biological Process: axon extension involved in axon guidance; axon guidance; branching morphogenesis of a tube; cell migration during sprouting angiogenesis; cellular response to hormone stimulus; chemorepulsion involved in embryonic olfactory bulb interneuron precursor migration; chemorepulsion involved in postnatal olfactory bulb interneuron migration; corticospinal neuron axon guidance through spinal cord; induction of negative chemotaxis; motor axon guidance; negative chemotaxis; negative regulation of actin filament polymerization; negative regulation of cell growth; negative regulation of cell migration; negative regulation of leukocyte chemotaxis; negative regulation of protein phosphorylation; negative regulation of small GTPase mediated signal transduction; negative regulation of smooth muscle cell migration; negative regulation of vascular permeability; positive regulation of apoptosis; positive regulation of axonogenesis; response to cortisol; retinal ganglion cell axon guidance; ureteric bud development

48-Strip-Wells

405 USD

96-Strip-Wells

675

5x96-Strip-Wells

3080

10x96-Strip-Wells

5805