catalog number :
MBS282883
products type :
ELISA Kit
products full name :
Rabbit Tumor necrosis factor alpha (TNFalpha) ELISA Kit
products short name :
[Tumor necrosis factor alpha]
other names :
[tumor necrosis factor alpha; tumor necrosis factor alpha]
products gene name :
[TNFalpha]
other gene names :
[tnfa]
specificity :
This assay has high sensitivity and excellent specificity for detection of Rabbit TNF-alpha. No significant cross-reactivity or interference between Rabbit TNF-alpha and analogues was observed.
storage stability :
Store at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 78.1 pg/mL - 5000 pg/mL
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: For the quantitative detection of Rabbit Tumor necrosis factor (TNF/TNFA/ TNFSF2) concentration in serum, plasma, cell culture supernates and other biological fluids. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate TNF-alpha in Rabbit serum, plasma, cell culture supernates. An antibody specific for TNF-alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNF-alpha is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF-alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001121579.1
ncbi gb acc num :
NM_001128107.1
size4 :
10x96-Strip-Wells