catalog number :
MBS282721
products type :
ELISA Kit
products full name :
Human Pleckstrin homology domain-containing family H member 3 (PLEKHH3) ELISA Kit
products short name :
Pleckstrin homology domain-containing family H member 3
other names :
pleckstrin homology domain-containing family H member 3; Pleckstrin homology domain-containing family H member 3; pleckstrin homology domain-containing family H member 3; PH domain-containing family H member 3; pleckstrin homology domain containing, family H (with MyTH4 domain) member 3
products gene name :
PLEKHH3
other gene names :
PLEKHH3; PLEKHH3; PH domain-containing family H member 3
uniprot entry name :
PKHH3_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human PLEKHH3. No significant cross-reactivity or interference between Human PLEKHH3 and analogues was observed.
storage stability :
Store at 2-8 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25 pg/mL - 2000 pg/mL. Sensitivity: The limit of detection of Human PLEKHH3 defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 15.6 pg/mL (mean of 6 independent assays).
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%. Intended Uses: For the quantitative detection of Human Pleckstrin homology domaincontaining family H member 3 (PLEKHH3) concentration in serum, plasma and other biological fluids.
products description :
Principle of the assay: This assay employs a two-site sandwich ELISA to quantitative PLEKHH3 in Human serum, plasma. An antibody specific for PLEKHH3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLEKHH3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for PLEKHH3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLEKHH3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_079203.3
ncbi gb acc num :
NM_024927.4
ncbi mol weight :
66,686 Da
uniprot summary :
PLEKHH3: 4 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 17q21.2. Cellular Component: extracellular space; cytoskeleton. Biological Process: signal transduction