ELISA/assay

Hamster Putative phospholipase B-like 2 (PLBD2) ELISA Kit

MBS282644

48-Strip-Wells

440 USD

ELISA Kit

Hamster Putative phospholipase B-like 2 (PLBD2) ELISA Kit

[Putative phospholipase B-like 2]

[putative phospholipase B-like 2 isoform 1; Putative phospholipase B-like 2; putative phospholipase B-like 2; PLB homolog 2; 76 kDa protein; LAMA-like protein 2; lamina ancestor homolog 2; phospholipase B-like 2 32 kDa form; phospholipase B-like 2 45 kDa form; phospholipase B domain-containing protein 2; mannose-6-phosphate protein associated protein p76; phospholipase B domain containing 2; 76 kDa protein; p76; LAMA-like protein 2; Lamina ancestor homolog 2; Phospholipase B domain-containing protein 2Cleaved into the following 2 chains:Putative phospholipase B-like 2 32 kDa form; Putative phospholipase B-like 2 45 kDa form]

[PLBD2]

[PLBD2; PLBD2; P76; p76]

PLBL2_HUMAN

Hamster

589

This assay has high sensitivity and excellent specificity for detection of Hamster (Mesocricetus; Cricetulus)PLBD2. No significant cross-reactivity or interference between Hamster (Mesocricetus; Cricetulus)PLBD2 and analogues was observed.

Store at 2-8 degree C.

Samples: Serum, Plasma, Other biological fluids. Assay Type: Sandwich

Sample Volume: 1-200 uL. Precision: Intra-assay Precision (Precision within an assay). Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays). Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Intra-Assay: CV. Inter-Assay: CV. Detection Wavelength: 450 nm

Most soluble lysosomal proteins carry Man6P (mannose 6-phosphate), a specific carbohydrate marker that enables their binding to cellular MPRs (Man6P receptors) and their subsequent targeting towards the lysosome. This characteristic was exploited to identify novel soluble lysosomal proteins by proteomic analysis of Man6P proteins purified from a human cell line. The presence of Man6P sugars was confirmed by an MPR overlay experiment, which showed the direct and Man6P-dependent interaction between p76 and the MPR. The presence of six N-glycosylation sites was validated by progressive peptide-N-glycosidase F deglycosylation. Experiments using N- and C-termini directed anti-p76 antibodies provided insights into p76 maturation. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PLBD2 in samples. An antibody specific for PLBD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLBD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLBD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLBD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

229093316

NP_775813.2

NM_173542.3

61,885 Da

PLBL2: Putative phospholipase. Belongs to the phospholipase B-like family. Protein type: Phospholipase; EC 3.1.1.-. Chromosomal Location of Human Ortholog: 12q24.13. Cellular Component: lysosomal lumen. Molecular Function: hydrolase activity. Biological Process: lipid catabolic process

48-Strip-Wells

440 USD

96-Strip-Wells

745

5x96-Strip-Wells

3405

10x96-Strip-Wells

6425