catalog number :
MBS282432
products type :
ELISA Kit
products full name :
Horse Osteocalcin (BGLAP) ELISA Kit
products short name :
[Osteocalcin]
other names :
[Osteocalcin; Osteocalcin; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein]
products gene name :
[BGLAP]
other gene names :
[BGLAP; BGP]
specificity :
This assay has high sensitivity and excellent specificity for detection of Human ATP13A2. No significant cross-reactivity or interference between Human ATP13A2 and analogues was observed.
storage stability :
Store at 2-8 degree C.
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 0.625 ng/mL-40 ng/mL. Sensitivity: 0.31 ng/mL (mean of 6 independent assays).
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ATP13A2 in Human serum, plasma. An antibody specific for ATP13A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ATP13A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATP13A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATP13A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
5,732 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
size4 :
10x96-Strip-Wells