product summary
Loading...
company name :
MyBioSource
product type :
ELISA/assay
product name :
Human 11-Deoxycortisol ELISA Kit
catalog :
MBS282369
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
product information
catalog number :
MBS282369
products type :
ELISA Kit
products full name :
Human 11-Deoxycortisol ELISA Kit
products short name :
11-Deoxycortisol
reactivity :
Human
specificity :
This assay has high sensitivity and excellent specificity for detection of Human 11-Deoxycortisol. No significant cross-reactivity or interference between Human 11-Deoxycortisol and analogues was observed.
storage stability :
Store at 2-8 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Competitive. Detection Range: 1.25 pmol/mL - 20 pmol/mL.The standard curve concentrations used for the ELISA's were 20 pmol/mL, 10 pmol/mL, 5 pmol/mL, 2.5 pmol/mL, 1.25 pmol/mL,. Sensitivity: The limit of detection of human 11-Deoxycortisol defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 0.625 pmol/mL (mean of 6 independent assays).
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Intended Uses: For the quantitative detection of Human 11-Deoxycortisol concentration in serum, plasma and other biological fluids.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 11-Deoxycortisol. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated 11-Deoxycortisol. A competitive inhibition reaction is launched between 11-Deoxycortisol (Standards or samples) and biotin-conjugated 11-Deoxycortisol with the pre-coated antibody specific for 11-Deoxycortisol. The more amount of 11-Deoxycortisol in samples, the less antibody bound by biotin-conjugated 11-Deoxycortisol. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of 11-Deoxycortisol in the sample. The color development is stopped and the intensity of the color is measured.
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
870
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!