catalog number :
MBS282369
products type :
ELISA Kit
products full name :
Human 11-Deoxycortisol ELISA Kit
products short name :
11-Deoxycortisol
specificity :
This assay has high sensitivity and excellent specificity for detection of Human 11-Deoxycortisol. No significant cross-reactivity or interference between Human 11-Deoxycortisol and analogues was observed.
storage stability :
Store at 2-8 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Competitive. Detection Range: 1.25 pmol/mL - 20 pmol/mL.The standard curve concentrations used for the ELISA's were 20 pmol/mL, 10 pmol/mL, 5 pmol/mL, 2.5 pmol/mL, 1.25 pmol/mL,. Sensitivity: The limit of detection of human 11-Deoxycortisol defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 0.625 pmol/mL (mean of 6 independent assays).
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Intended Uses: For the quantitative detection of Human 11-Deoxycortisol concentration in serum, plasma and other biological fluids.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 11-Deoxycortisol. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated 11-Deoxycortisol. A competitive inhibition reaction is launched between 11-Deoxycortisol (Standards or samples) and biotin-conjugated 11-Deoxycortisol with the pre-coated antibody specific for 11-Deoxycortisol. The more amount of 11-Deoxycortisol in samples, the less antibody bound by biotin-conjugated 11-Deoxycortisol. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of 11-Deoxycortisol in the sample. The color development is stopped and the intensity of the color is measured.