ELISA/assay

Mouse XIAP-associated factor 1 (XAF1) ELISA Kit

MBS282356

48-Strip-Wells

405 USD

ELISA Kit

Mouse XIAP-associated factor 1 (XAF1) ELISA Kit

[XIAP-associated factor 1]

[XIAP-associated factor 1 isoform 1; XIAP-associated factor 1; XIAP-associated factor 1; XIAP associated factor 1; BIRC4-binding protein]

[XAF1]

[Xaf1; Xaf1; Fbox39; Birc4bp; Xiapaf1]

Mouse

301

This assay has high sensitivity and excellent specificity for detection of Mouse XAF1. No significant cross-reactivity or interference between Mouse XAF1 and analogues was observed.

Store at 2-8 degree C.

Samples: serum, plasma and other biological fluids. Detection Range: 15.6 pg/mL - 1000 pg/mL. Sensitivity: 7.8 pg/mL (mean of 6 independent assays).

Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: For the quantitative detection of Mouse XIAP-associated factor 1 (XAF1) concentration in serum, plasma and other biological fluids. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate XAF1 in Mouse serum, plasma. An antibody specific for XAF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any XAF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XAF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XAF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

161333845

NP_001032802.2

NM_001037713.4

Q5NBU8

20,465 Da

BIRC4BP: Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti- caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha- induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Apoptosis; Tumor suppressor. Chromosomal Location of Human Ortholog: 11 11 B4. Cellular Component: cytoplasm; mitochondrion; nucleus. Molecular Function: metal ion binding. Biological Process: apoptosis; negative regulation of protein complex assembly; response to cytokine

48-Strip-Wells

405 USD

96-Strip-Wells

675

5x96-Strip-Wells

3080

10x96-Strip-Wells

5805