ELISA/assay

Human Probable methylthioribulose-1-phosphate dehydratase (APIP) ELISA Kit

MBS282228

48-Strip-Wells

405 USD

ELISA Kit

Human Probable methylthioribulose-1-phosphate dehydratase (APIP) ELISA Kit

[Probable methylthioribulose-1-phosphate dehydratase]

[methylthioribulose-1-phosphate dehydratase; Methylthioribulose-1-phosphate dehydratase; methylthioribulose-1-phosphate dehydratase; APAF1 interacting protein; APAF1-interacting protein]

[APIP]

[APIP; APIP; APIP2; CGI29; hAPIP; CGI-29; MMRP19; hAPIP]

Human

240

This assay has high sensitivity and excellent specificity for detection of Human APIP. No significant cross-reactivity or interference between Human APIP and analogues was observed.

Store at 2-8 degree C.

Samples: Serum, Plasma, Other biological fluids. Assay Type: Sandwich

Sample Volume: 1-200 uL. Precision: Intra-assay Precision (Precision within an assay). Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays). Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Intra-Assay: CV. Inter-Assay: CV. Detection Wavelength: 450 nm

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate APIP in samples. An antibody specific for APIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAPIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APIP bound in the initial step. The color development is stopped and the intensity of the color is measured.

166235186

NP_057041.2

NM_015957.3

Q96GX9

22,858 Da

Cysteine And Methionine Metabolism Pathway (104488); Cysteine And Methionine Metabolism Pathway (103421); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Metabolism Of Polyamines Pathway (106193); Methionine Salvage Pathway (413446); Methionine Salvage Pathway (366245); Methionine Salvage Pathway (468227); S-methyl-5-thio-alpha-D-ribose 1-phosphate Degradation Pathway (547487)

APIP is an APAF1 (MIM 602233)-interacting protein that acts as a negative regulator of ischemic/hypoxic injury (Cho et al., 2004 [PubMed 15262985]).[supplied by OMIM, Dec 2008]

MMRP19: Catalyzes the dehydration of methylthioribulose-1- phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1- phosphate (DK-MTP-1-P). Has an anti-apoptotic function and prevents muscle ischemic damage. Inhibits the cytochrome c- dependent and APAF1-mediated cell death. Belongs to the aldolase class II family. MtnB subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Amino Acid Metabolism - cysteine and methionine; Apoptosis; EC 4.2.1.109. Chromosomal Location of Human Ortholog: 11p13. Cellular Component: cytoplasm. Molecular Function: identical protein binding; methylthioribulose 1-phosphate dehydratase activity; protein binding; zinc ion binding. Biological Process: apoptosis; L-methionine salvage from methylthioadenosine; negative regulation of apoptosis; protein homotetramerization; pyroptosis; regulation of ERK1 and ERK2 cascade

48-Strip-Wells

405 USD

96-Strip-Wells

675

5x96-Strip-Wells

3080

10x96-Strip-Wells

5805