ELISA/assay

Human Prestin (SLC26A5) ELISA Kit

MBS282125

48-Strip-Wells

405 USD

ELISA Kit

Human Prestin (SLC26A5) ELISA Kit

[Prestin]

[prestin isoform e; Prestin; prestin; solute carrier family 26 member 5; Solute carrier family 26 member 5]

[SLC26A5]

[SLC26A5; SLC26A5; PRES; DFNB61; PRES]

Human

744

This assay has high sensitivity and excellent specificity for detection of Human RBM22. No significant cross-reactivity or interference between Human RBM22 and analogues was observed.

Store at 2-8 degree C.

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 62.5 pg/mL-4000 pg/mL. Sensitivity: 20.8 pg/mL (mean of 6 independent assays).

Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: For the quantitative detection of Human Pre-mRNA-splicing factor RBM22 (RBM22) concentration in serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate RBM22 in Human serum, plasma, tissue homogenates. An antibody specific for RBM22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RBM22 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RBM22 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBM22 bound in the initial step. The color development is stopped and the intensity of the color is measured.

269784651

NP_001161434.1

NM_001167962.1

P58743

77,809 Da

This gene encodes a member of the SLC26A/SulP transporter family. The protein functions as a molecular motor in motile outer hair cells (OHCs) of the cochlea, inducing changes in cell length that act to amplify sound levels. The transmembrane protein is an incomplete anion transporter, and does not allow anions to cross the cell membrane but instead undergoes a conformational change in response to changes in intracellular Cl- levels that results in a change in cell length. The protein functions at microsecond rates, which is several orders of magnitude faster than conventional molecular motor proteins. Mutations in this gene are potential candidates for causing neurosensory deafness. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Nov 2009]

SLC26A5: Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage- to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site. Defects in SLC26A5 are the cause of deafness autosomal recessive type 61 (DFNB61). A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the SLC26A/SulP transporter (TC 2.A.53) family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, SLC family. Chromosomal Location of Human Ortholog: 7q22.1. Cellular Component: basolateral plasma membrane; cytoplasm; integral component of plasma membrane; lateral plasma membrane. Molecular Function: anion:anion antiporter activity; bicarbonate transmembrane transporter activity; chloride channel activity; oxalate transmembrane transporter activity; protein homodimerization activity; secondary active sulfate transmembrane transporter activity; spectrin binding; sulfate transmembrane transporter activity; transcription factor binding. Biological Process: bicarbonate transport; fructose transport; oxalate transport; positive regulation of cell size; protein tetramerization; regulation of cell shape; regulation of intracellular pH; regulation of membrane potential; response to drug; response to salicylic acid; sensory perception of sound

48-Strip-Wells

405 USD

96-Strip-Wells

675

5x96-Strip-Wells

3080

10x96-Strip-Wells

5805