catalog number :
MBS282053
products type :
ELISA Kit
products full name :
Bovine Growth hormone (GH) ELISA Kit
products short name :
[Growth hormone]
other names :
[somatotropin; Somatotropin; somatotropin; Growth hormone]
products gene name :
[GH]
other gene names :
[GH1; GH1; GH; GH]
specificity :
This assay has high sensitivity and excellent specificity for detection of Bovine GH1. No significant cross-reactivity or interference between Bovine GH1 and analogues was observed.
storage stability :
Store at 2-8 degree C.
other info1 :
Samples: Serum, Plasma, Other biological fluids. Assay Type: Sandwich
other info2 :
Sample Volume: 1-200 uL. Precision: Intra-assay Precision (Precision within an assay). Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays). Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Intra-Assay: CV. Inter-Assay: CV. Detection Wavelength: 450 nm
products description :
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GH1 in samples. An antibody specific for GH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_851339.1
ncbi gb acc num :
NM_180996.1
ncbi mol weight :
24,558 Da
ncbi pathways :
Cytokine Signaling In Immune System Pathway (1119410); Growth Hormone Receptor Signaling Pathway (1119427); Immune System Pathway (1119286); Jak-STAT Signaling Pathway (84316); Jak-STAT Signaling Pathway (488); Metabolism Of Proteins Pathway (1119622); Neuroactive Ligand-receptor Interaction Pathway (84292); Neuroactive Ligand-receptor Interaction Pathway (462); Nuclear Signaling By ERBB4 Pathway (1120138); PI3K-Akt Signaling Pathway (692236)
uniprot summary :
Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
size4 :
10x96-Strip-Wells