catalog number :
MBS281832
products type :
ELISA Kit
products full name :
Rat Interleukin 1beta (IL-1beta) ELISA Kit
products short name :
[Interleukin 1beta]
other names :
[interleukin-1 beta; Interleukin-1 beta; interleukin-1 beta; Lymphocyte proliferation-potentiating factor]
products gene name :
[IL-1beta]
other gene names :
[IL1B; IL1B; IL1-BETA; IL-1 beta]
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat IL-8. No significant cross-reactivity or interference between Rat IL-8 and analogues was observed.
storage stability :
Store at 2-8 degree C.
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 3.12 pg/mL-200 pg/mL. Sensitivity: 1.56 pg/mL (mean of 6 independent assays).
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: For the quantitative detection of Rat Interleukin 8 (IL-8) concentration in serum, plasma and other biological fluids. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IL-8 in Rat serum, plasma. An antibody specific for IL-8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-8 present is bound by the immobilized antibody. After removing any unbound substances, a biotinconjugated antibody specific for IL-8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001075670.1
ncbi gb acc num :
NM_001082201.1
ncbi mol weight :
30,665 Da
uniprot summary :
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
size4 :
10x96-Strip-Wells