catalog number :
MBS281820
products type :
ELISA Kit
products full name :
Fish 17-alpha Methyltestosterone (17-alphaMT) ELISA Kit
products short name :
17-alpha Methyltestosterone
products gene name :
17-alphaMT
specificity :
This assay has high sensitivity and excellent specificity for detection of Fish 17-alphaMT. No significant cross-reactivity or interference between Fish 17-alphaMT and analogues was observed.
storage stability :
Store at 2-8 degree C.
other info1 :
Samples: Serum and plasma medical treated fish and other biological fluids. Assay Type: Competitive. Detection Range: 0.5 ng/mL - 100 ng/mL.The standard curve concentrations used for the ELISA's were 100 ng/mL, 25 ng/mL, 8 ng/mL, 2 ng/mL, 0.5 ng/mL, 0 ng/mL. Sensitivity: The limit of detection of Fish 17-alphaMT defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 0.25 ng/mL (mean of 6 independent assays).
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Intended Uses: For the quantitative detection of Fish 17-alpha Methyltestosterone (17-alphaMT) concentration in serum and plasma medical treated fish and other biological fluids.
products description :
Principle of the assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for 17-alphaMT and Horseradish Peroxidase (HRP) conjugated 17-alphaMT. The competitive inhibition reaction is launched between with HRP labeled 17-alphaMT and unlabeled 17-alphaMT with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of 17-alphaMT in the sample. The color development is stopped and the intensity of the color is measured.