catalog number :
MBS281782
products type :
ELISA Kit
products full name :
Chicken Luteinizing Hormone (LH) ELISA Kit
products short name :
[Luteinizing Hormone]
products gene name :
[LH]
specificity :
This assay has high sensitivity and excellent specificity for detection of Chicken LH. No significant cross-reactivity or interference between Chicken LH and analogues was observed.
storage stability :
Store at 2-8 degree C.
other info1 :
Samples: Serum, Plasma, Other biological fluids. Assay Type: Sandwich
other info2 :
Sample Volume: 1-200 uL. Precision: Intra-assay Precision (Precision within an assay). Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays). Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Intra-Assay: CV. Inter-Assay: CV. Detection Wavelength: 450 nm
products description :
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate LH in samples. An antibody specific for LH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LH bound in the initial step. The color development is stopped and the intensity of the color is measured.
size4 :
10x96-Strip-Wells