catalog number :
MBS281756
products type :
ELISA Kit
products full name :
Human Pre-rRNA-processing protein TSR1 homolog (TSR1) ELISA Kit
products short name :
Pre-rRNA-processing protein TSR1 homolog
other names :
pre-rRNA-processing protein TSR1 homolog; Pre-rRNA-processing protein TSR1 homolog; pre-rRNA-processing protein TSR1 homolog; TSR1, 20S rRNA accumulation, homolog (S. cerevisiae)
products gene name :
TSR1
other gene names :
TSR1; TSR1; KIAA1401
uniprot entry name :
TSR1_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human TSR1. No significant cross-reactivity or interference between Human TSR1 and analogues was observed.
storage stability :
Store at 2-8 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 1.56 ng/mL - 100 ng/mL. Sensitivity: The limit of detection of Human TSR1 defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 0.78 ng/mL (mean of 6 independent assays).
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%. Intended Uses: For the quantitative detection of Human Pre-rRNA-processing protein TSR1 homolog (TSR1) concentration in serum, plasma and other biological fluids.
products description :
Principle of the assay: This assay employs a two-site sandwich ELISA to quantitative TSR1 in Human serum, plasma. An antibody specific for TSR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for TSR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_060598.3
ncbi gb acc num :
NM_018128.4
ncbi mol weight :
91,810 Da
uniprot summary :
TSR1: Required during maturation of the 40S ribosomal subunit in the nucleolus. Belongs to the BMS1/TSR1 family. TSR1 subfamily. Protein type: Nucleolus. Chromosomal Location of Human Ortholog: 17p13.3. Cellular Component: nucleolus. Biological Process: ribosome assembly