Human Interleukin 18 (IL-18) ELISA Kit | MyBioSource MBS281497 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Interleukin 18 (IL-18) ELISA Kit

catalog :

MBS281497

quantity :

48-Strip-Wells

price :

340 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS281497

products type :

ELISA Kit

products full name :

Human Interleukin 18 (IL-18) ELISA Kit

products short name :

[Interleukin 18]

other names :

[interleukin-18 isoform 2; Interleukin-18; interleukin-18; interleukin 18; Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma]

products gene name :

[IL-18]

other gene names :

[IL18; IL18; IGIF; IL-18; IL-1g; IL1F4; IGIF; IL1F4; IL-18; IFN-gamma-inducing factor; IL-1 gamma]

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of Human IL-18. No significant cross-reactivity or interference between Human IL-18 and analogues was observed.

storage stability :

Store at 2-8 degree C.

other info1 :

Samples: serum, plasma, urine, saliva and other biological fluids. Detection Range: 15.6 pg/mL - 1000 pg/mL. Sensitivity: 5.2 pg/mL (mean of 6 independent assays).

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: For the quantitative detection of Human Interleukin 18 (IL-18) concentration in serum, plasma, urine, saliva and other biological fluids. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IL-18 in Human serum, plasma, urine, saliva. An antibody specific for IL-18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-18 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-18 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-18 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

342349321

ncbi acc num :

NP_001230140.1

ncbi gb acc num :

NM_001243211.1

uniprot acc num :

Q14116

ncbi mol weight :

21,896 Da

ncbi pathways :

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Cellular Roles Of Anthrax Toxin Pathway (138076); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); IL12 Signaling Mediated By STAT4 Pathway (137936); IL12-mediated Signaling Events Pathway (137922)

ncbi summary :

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

uniprot summary :

IL18: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Belongs to the IL-1 family. Protein type: Cytokine. Chromosomal Location of Human Ortholog: 11q23.1. Cellular Component: cytosol; extracellular region; extracellular space. Molecular Function: cytokine activity; protein binding. Biological Process: activation of protein kinase B activity; angiogenesis; cell-cell signaling; chemokine biosynthetic process; cholesterol homeostasis; granulocyte macrophage colony-stimulating factor biosynthetic process; immune response; inflammatory response; interferon-gamma biosynthetic process; interleukin-13 biosynthetic process; interleukin-2 biosynthetic process; lipopolysaccharide-mediated signaling pathway; MAPK cascade; natural killer cell activation; negative regulation of myoblast differentiation; positive regulation of activated T cell proliferation; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of inflammatory response; positive regulation of interferon-gamma production; positive regulation of interleukin-17 production; positive regulation of natural killer cell proliferation; positive regulation of NF-kappaB import into nucleus; positive regulation of NK T cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling; positive regulation of smooth muscle cell proliferation; positive regulation of T-helper 2 cell differentiation; positive regulation of tissue remodeling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of STAT protein; regulation of cell adhesion; sleep; T-helper 1 type immune response; type 2 immune response

size1 :

48-Strip-Wells

price1 :

340 USD

size2 :

96-Strip-Wells

price2 :

555

size3 :

5x96-Strip-Wells

price3 :

2490

size4 :

10x96-Strip-Wells

price4 :

4685

more info or order :

MyBioSource product webpage