ELISA/assay

Human Interleukin 1beta (IL-1beta) ELISA Kit

MBS281439

48-Strip-Wells

285 USD

ELISA Kit

Human Interleukin 1beta (IL-1beta) ELISA Kit

[Interleukin 1beta]

[interleukin-1 beta proprotein; Interleukin-1 beta; interleukin-1 beta; interleukin 1 beta; Catabolin]

[IL-1beta]

[IL1B; IL1B; IL-1; IL1F2; IL1-BETA; IL1F2; IL-1 beta]

Human

267

This assay has high sensitivity and excellent specificity for detection of Human IL1B. No significant cross-reactivity or interference between Human IL1B and analogues was observed.

Store at 2-8 degree C.

Samples: Serum, Plasma, Other biological fluids. Assay Type: Sandwich

Sample Volume: 1-200 uL. Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.CV (%) = SD/meanX100Intra-Assay: CV<8% Inter-Assay: CV<12%. Detection Wavelength: 450 nm

Interleukin-1 (IL-1) is one of the first cytokines ever described. Its initial discovery was as a factor that could induce fever, control lymphocytes, increase the number of bone marrow cells and cause degeneration of bone joints. At this time, IL-1 was known under several other names including endogenous pyrogen, lymphocyte activating factor, haemopoetin-1 and mononuclear cell factor, amongst others. It was around 1984-1985 when scientists confirmed that IL-1 was actually composed of two distinct proteins, now called IL-1. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IL1B in samples. An antibody specific for IL1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL1B bound in the initial step. The color development is stopped and the intensity of the color is measured.

10835145

NP_000567.1

NM_000576.2

P01584

30,748 Da

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Apoptosis Pathway (83060); Apoptosis Pathway (470); Cellular Roles Of Anthrax Toxin Pathway (138076)

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]

IL1B: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Monomer. Belongs to the IL-1 family. Protein type: Cytokine. Chromosomal Location of Human Ortholog: 2q14.1. Cellular Component: cytosol; extracellular exosome; extracellular region; extracellular space; lysosome; secretory granule. Molecular Function: cytokine activity; interleukin-1 receptor binding; protein binding; protein domain specific binding. Biological Process: activation of MAPK activity; apoptosis; cell-cell signaling; cellular response to organic cyclic compound; cellular response to organic substance; cytokine-mediated signaling pathway; embryo implantation; fever; germ cell programmed cell death; hyaluronan biosynthetic process; immune response; inflammatory response; interleukin-1 beta production; interleukin-6 production; lipopolysaccharide-mediated signaling pathway; MAPK cascade; negative regulation of cell proliferation; negative regulation of extrinsic apoptotic signaling pathway in absence of ligand; negative regulation of glucose transport; negative regulation of insulin receptor signaling pathway; negative regulation of lipid catabolic process; negative regulation of lipid metabolic process; negative regulation of MAP kinase activity; negative regulation of synaptic transmission; neutrophil chemotaxis; positive regulation of angiogenesis; positive regulation of cell division; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of chemokine biosynthetic process; positive regulation of epithelial to mesenchymal transition; positive regulation of fever; positive regulation of gene expression; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of heterotypic cell-cell adhesion; positive regulation of histone acetylation; positive regulation of histone phosphorylation; positive regulation of interferon-gamma production; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-6 biosynthetic process; positive regulation of interleukin-6 production; positive regulation of interleukin-6 secretion; positive regulation of interleukin-8 production; positive regulation of JNK cascade; positive regulation of lipid catabolic process; positive regulation of membrane protein ectodomain proteolysis; positive regulation of mitotic nuclear division; positive regulation of monocyte chemotactic protein-1 production; positive regulation of NF-kappaB import into nucleus; positive regulation of NF-kappaB transcription factor activity; positive regulation of nitric oxide biosynthetic process; positive regulation of p38MAPK cascade; positive regulation of phagocytosis; positive regulation of prostaglandin secretion; positive regulation of protein export from nucleus; positive regulation of protein phosphorylation; positive regulation of T cell mediated immunity; positive regulation of T cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; positive regulation of vascular endothelial growth factor production; positive regulation of vascular endothelial growth factor receptor signaling pathway; protein kinase B signaling; regulation of defense response to virus by host; regulation of ERK1 and ERK2 cascade; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of insulin secretion; regulation of nitric-oxide synthase activity; regulation of receptor activity; response to ATP; response to carbohydrate stimulus; sequestering of triacylglycerol; signal transduction; smooth muscle adaptation. Disease: Gastric Cancer, Hereditary Diffuse

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440

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1925

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3620