catalog number :
MBS281220
products type :
ELISA Kit
products full name :
Human Phosphorylated Myosin Light Chain (PMLC) ELISA Kit
products short name :
Phosphorylated Myosin Light Chain
products gene name :
PMLC
specificity :
This assay has high sensitivity and excellent specificity for detection of Human PMLC. No significant cross-reactivity or interference between Human PMLC and analogues was observed.
storage stability :
Store at 2-8 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.78 ng/mL - 50 ng/mL. Sensitivity: The limit of detection of Human PMLC defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 0.39 ng/mL (mean of 6 independent assays).
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%. Intended Uses: For the quantitative detection of Human Phosphorylated Myosin Light Chain (PMLC) concentration in serum, plasma and other biological fluids.
products description :
Principle of the assay: This assay employs a two-site sandwich ELISA to quantitative PMLC in Human serum, plasma. An antibody specific for PMLC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PMLC present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for PMLC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PMLC bound in the initial step. The color development is stopped and the intensity of the color is measured.