ELISA/assay

Human Protein Wnt-3a (WNT3A) ELISA Kit

MBS281216

48-Strip-Wells

405 USD

ELISA Kit

Human Protein Wnt-3a (WNT3A) ELISA Kit

[Protein Wnt-3a]

[protein Wnt-3a; Protein Wnt-3a; protein Wnt-3a; Wnt family member 3A]

[WNT3A]

[WNT3A; WNT3A]

Human

352

This assay has high sensitivity and excellent specificity for detection of Human WNT2. No significant cross-reactivity or interference between Human WNT2 and analogues was observed.

Store at 2-8 degree C.

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156 ng/mL-10 ng/mL. Sensitivity: 0.078 ng/mL (mean of 6 independent assays).

Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: For the quantitative detection of Human Protein Wnt-2 (WNT2) concentration in serum, plasma and other biological fluids. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate WNT2 in Human serum, plasma. An antibody specific for WNT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

14916475

NP_149122.1

NM_033131.3

P56704

42,905 Da

Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Canonical Wnt Signaling Pathway (138032); Cardiac Progenitor Differentiation Pathway (712094); Class B/2 (Secretin Family Receptors) Pathway (106378); DNA Damage Response (only ATM Dependent) Pathway (198827); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); GPCR Ligand Binding Pathway (161020); HTLV-I Infection Pathway (373901)

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]

WNT3A: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6. Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. Belongs to the Wnt family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q42.13. Cellular Component: cell surface; early endosome membrane; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane; proteinaceous extracellular matrix. Molecular Function: frizzled binding; frizzled-2 binding; protein binding; protein domain specific binding; receptor binding; receptor ligand activity; transcription coactivator activity. Biological Process: axon guidance; cell proliferation in forebrain; cell proliferation in midbrain; COP9 signalosome assembly; dorsal/ventral neural tube patterning; extracellular matrix organization; heart looping; hemopoiesis; hippocampus development; in utero embryonic development; inner ear morphogenesis; mammary gland development; negative regulation of axon extension involved in axon guidance; negative regulation of fat cell differentiation; negative regulation of neurogenesis; neuron differentiation; osteoblast differentiation; palate development; paraxial mesodermal cell fate commitment; positive regulation of B cell proliferation; positive regulation of cell proliferation; positive regulation of collateral sprouting in the absence of injury; positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; positive regulation of cytokine production; positive regulation of mesodermal cell fate specification; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein binding; positive regulation of protein phosphorylation; positive regulation of receptor internalization; positive regulation of skeletal muscle tissue development; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; spinal cord association neuron differentiation; Wnt receptor signaling pathway through beta-catenin; Wnt signaling pathway; Wnt signaling pathway involved in forebrain neuroblast division

48-Strip-Wells

405 USD

96-Strip-Wells

675

5x96-Strip-Wells

3080

10x96-Strip-Wells

5805