This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Pleckstrin (PLEK) ELISA Kit
catalog :
MBS281194
quantity :
48-Strip-Wells
price :
405 USD
product information
catalog number :
MBS281194
products type :
ELISA Kit
products full name :
Human Pleckstrin (PLEK) ELISA Kit
products short name :
[Pleckstrin]
other names :
[pleckstrin; Pleckstrin; pleckstrin; pleckstrin; Platelet 47 kDa protein; p47]
products gene name :
[PLEK]
other gene names :
[PLEK; PLEK; P47; P47; p47]
reactivity :
Human
sequence length :
350
specificity :
This assay has high sensitivity and excellent specificity for detection of Human PDGFD. No significant cross-reactivity or interference between Human PDGFD and analogues was observed.
storage stability :
Store at 2-8 degree C.
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.31 ng/mL-20 ng/mL. Sensitivity: 0.156 ng/mL (mean of 6 independent assays).
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: For the quantitative detection of Human Platelet-derived growth factor D (PDGFD) concentration in serum, plasma and other biological fluids. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PDGFD in Human serum, plasma. An antibody specific for PDGFD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDGFD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGFD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGFD bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
156616273
ncbi acc num :
NP_002655.2
ncbi gb acc num :
NM_002664.2
uniprot acc num :
P08567
ncbi mol weight :
40,125 Da
ncbi pathways :
Hemostasis Pathway (106028); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048)
uniprot summary :
pleckstrin: Major protein kinase C substrate of platelets. Protein type: Lipid-binding. Chromosomal Location of Human Ortholog: 2p14. Cellular Component: cytoplasm; cytosol; extracellular region; membrane. Molecular Function: phosphatidylinositol-3,4-bisphosphate binding; protein binding; protein homodimerization activity; protein kinase C binding. Biological Process: actin cytoskeleton reorganization; cell projection organization; cortical actin cytoskeleton organization and biogenesis; hemopoietic progenitor cell differentiation; integrin-mediated signaling pathway; negative regulation of calcium-mediated signaling; negative regulation of G-protein coupled receptor protein signaling pathway; negative regulation of inositol phosphate biosynthetic process; phosphatidylinositol metabolic process; phospholipase C inhibition; platelet degranulation; positive regulation of actin filament bundle assembly; positive regulation of actin filament depolymerization; positive regulation of inositol-polyphosphate 5-phosphatase activity; positive regulation of integrin activation; positive regulation of platelet activation; ruffle organization; vesicle docking involved in exocytosis
size1 :
48-Strip-Wells
price1 :
405 USD
size2 :
96-Strip-Wells
price2 :
675
size3 :
5x96-Strip-Wells
price3 :
3080
size4 :
10x96-Strip-Wells
price4 :
5805
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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