catalog number :
MBS281137
products type :
ELISA Kit
products full name :
Fish Follicle-Stimulating Hormone (FSH) ELISA Kit
products short name :
Follicle-Stimulating Hormone
specificity :
This assay has high sensitivity and excellent specificity for detection of Fish FSH. No significant cross-reactivity or interference between Fish FSH and analogues was observed.
storage stability :
Store at 2-8 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Competitive. Detection Range: 8 mIU/mL - 240 mIU/mL.The standard curve concentrations used for the ELISA's were 240 mIU/mL, 96 mIU/mL, 48 mIU/mL, 24 mIU/mL, 8 mIU/mL, 0 mIU/mL. Sensitivity: 4 mIU/mL (mean of 6 independent assays).
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: For the quantitative detection of Fish Follicle-stimulating hormone (FSH) concentration in serum, plasma and other biological fluids. Principle of the Assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for FSH and Horseradish Peroxidase (HRP) conjugated FSH. The competitive inhibition reaction is launched between with HRP labeled FSH and unlabeled FSH with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of FSH in the sample. The color development is stopped and the intensity of the color is measured.