ELISA/assay

Human Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) ELISA Kit

MBS281111

48-Strip-Wells

485 USD

ELISA Kit

Human Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) ELISA Kit

Probable E3 ubiquitin-protein ligase makorin-3

probable E3 ubiquitin-protein ligase makorin-3; Probable E3 ubiquitin-protein ligase makorin-3; probable E3 ubiquitin-protein ligase makorin-3; RING finger protein 63; zinc finger protein 127; makorin ring finger protein 3; RING finger protein 63; Zinc finger protein 127

MKRN3

MKRN3; MKRN3; CPPB2; D15S9; RNF63; ZFP127; ZNF127; D15S9; RNF63; ZNF127

MKRN3_HUMAN

Human

507

This assay has high sensitivity and excellent specificity for detection of Human MKRN3. No significant cross-reactivity or interference between Human MKRN3 and analogues was observed.

Store at 2-8 degree C.

Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25 pg/mL - 2000 pg/mL. The standard curve concentrations used for the ELISA's were 2000 pg/mL, 1000 pg/mL, 500 pg/mL, 250 pg/mL, 125 pg/mL, 62.5 pg/mL, 31.25 pg/mL, 0 pg/mL. Sensitivity: 7.8 pg/mL (mean of 6 independent assays).

Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: For the quantitative detection of Human Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) concentration in serum, plasma, tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitative MKRN3 in Human serum, plasma, tissue homogenates, cell lysates. An antibody specific for MKRN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MKRN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for MKRN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MKRN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

5032243

NP_005655.1

NM_005664.3

55,645 Da

The protein encoded by this gene contains a RING (C3HC4) zinc finger motif and several C3H zinc finger motifs. This gene is intronless and imprinted, with expression only from the paternal allele. Disruption of the imprinting at this locus may contribute to Prader-Willi syndrome. An antisense RNA of unknown function has been found overlapping this gene. [provided by RefSeq, Jul 2008]

MKRN3: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Protein type: Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.-; EC 6.3.2.19. Chromosomal Location of Human Ortholog: 15q11-q13. Cellular Component: ribonucleoprotein complex. Molecular Function: protein binding; zinc ion binding; ligase activity. Biological Process: protein ubiquitination. Disease: Prader-willi Syndrome; Precocious Puberty, Central, 2

48-Strip-Wells

485 USD

96-Strip-Wells

820