ELISA/assay

Pig insulin-like growth factors-1 (IGF-1) ELISA Kit

MBS280894

48-Strip-Wells

385 USD

ELISA Kit

Pig insulin-like growth factors-1 (IGF-1) ELISA Kit

[insulin-like growth factors-1]

[insulin-like growth factor I; Insulin-like growth factor I; insulin-like growth factor I; Somatomedin]

[IGF-1]

[IGF1; IGF1; IGF-1; IGF-I; Npt2B; IGF-I]

Pig

154

This assay has high sensitivity and excellent specificity for detection of Pig IGF-1. No significant cross-reactivity or interference between Pig IGF-1 and analogues was observed.

Store at 2-8 degree C.

Samples: serum, plasma and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 3.12 ng/mL - 200 ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: For the quantitative detection of Pig Insulin-like growth factors-1 (IGF-1) concentration in serum, plasma and other biological fluids. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IGF-1 in Pig serum, plasma. An antibody specific for IGF-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGF-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGF-1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGF-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

47523588

NP_999421.1

NM_214256.1

P16545

17,010 Da

AMPK Signaling Pathway (989938); AMPK Signaling Pathway (992181); Aldosterone-regulated Sodium Reabsorption Pathway (130624); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Dilated Cardiomyopathy Pathway (121477); Dilated Cardiomyopathy Pathway (121285); Focal Adhesion Pathway (84479); Focal Adhesion Pathway (478); FoxO Signaling Pathway (921549); Glioma Pathway (84517)

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 ().

48-Strip-Wells

385 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5450