ELISA/assay

Human Probable G-protein coupled receptor 132 (GPR132) ELISA Kit

MBS280856

48-Strip-Wells

485 USD

ELISA Kit

Human Probable G-protein coupled receptor 132 (GPR132) ELISA Kit

Probable G-protein coupled receptor 132

probable G-protein coupled receptor 132 isoform 3; Probable G-protein coupled receptor 132; probable G-protein coupled receptor 132; G2 accumulation protein; G protein-coupled receptor 132; G2 accumulation protein

GPR132

GPR132; GPR132; G2A; G2A

GP132_HUMAN

Human

192

This assay has high sensitivity and excellent specificity for detection of Human GPR132. No significant cross-reactivity or interference between Human GPR132 and analogues was observed.

Store at 2-8 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 15.6 pg/mL - 1000 pg/mL. The standard curve concentrations used for the ELISA's were 1000 pg/mL, 500 pg/mL, 250 pg/mL, 125 pg/mL, 62.5 pg/mL, 31.25 pg/mL, 15.6 pg/mL, 0 pg/mL. Sensitivity: 7.8 pg/mL (mean of 6 independent assays).

Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: For the quantitative detection of Human Probable G-protein coupled receptor 132 (GPR132) concentration in serum, plasma and other biological fluids. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GPR132 in Human serum, plasma. An antibody specific for GPR132 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPR132 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPR132 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPR132 bound in the initial step. The color development is stopped and the intensity of the color is measured.

524580092

NP_001265625.1

NM_001278696.1

41,462 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Other Pathway (198765); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein was reported to be a receptor for lysophosphatidylcholine action, but PubMedID: 15653487 retracts this finding and instead suggests this protein to be an effector of lysophosphatidylcholine action. This protein may have proton-sensing activity and may be a receptor for oxidized free fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

GPR132: Receptor for an unknown ligand. Activates a G alpha protein, most likely G alpha(q). May be involved in apoptosis. Functions at the G2/M checkpoint to delay mitosis. May serve as a mechanism for T- and B-cells, and other cell types, to slow their proliferation and repair damaged DNA to ensure proper replication. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR. Chromosomal Location of Human Ortholog: 14q32.3. Cellular Component: integral to membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway; G1/S transition of mitotic cell cycle

48-Strip-Wells

485 USD

96-Strip-Wells

820