Goat Haptoglobin (HP) ELISA Kit | MyBioSource MBS280796 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Goat Haptoglobin (HP) ELISA Kit

catalog :

MBS280796

quantity :

48-Strip-Wells

price :

525 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS280796

products type :

ELISA Kit

products full name :

Goat Haptoglobin (HP) ELISA Kit

products short name :

Haptoglobin

other names :

haptoglobin; Haptoglobin; haptoglobin; zonulin; binding peptide; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; haptoglobin, beta polypeptide; haptoglobin, alpha polypeptide; haptoglobin; ZonulinCleaved into the following 2 chains:Haptoglobin alpha chain; Haptoglobin beta chain

products gene name :

other gene names :

HP; HP; BP; HPA1S; HP2ALPHA2

uniprot entry name :

HPT_HUMAN

reactivity :

Goat

sequence length :

406

specificity :

This assay has high sensitivity and excellent specificity for detection of Goat HP. No significant cross-reactivity or interference between Goat HP and analogues was observed.

storage stability :

Store at 2-8 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Detection Range: 15.6 ng/mL - 500 ng/mL. Sensitivity: The limit of detection of Goat HP defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 20 ng/mL (mean of 6 independent assays).

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%. Intended Uses: For the quantitative detection of Goat Haptoglobin (HP) concentration in serum, plasma and other biological fluids.

products description :

Principle of the assay: The microtiter plate provided in this kit has been pre-coated with Goat HP. Samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated antibody preparation specific for Goat HP and incubated. Then substrate solutions are added to each well. Only those wells that contain Goat HP and enzyme-conjugated Goat HP will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm.

ncbi gi num :

386783

ncbi acc num :

AAA88080.1

ncbi mol weight :

38,452 Da

ncbi pathways :

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Scavenging Of Heme From Plasma Pathway (771600); Amb2 Integrin Signaling Pathway (137945)

ncbi summary :

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. The protein encoded also exhibits antimicrobial activity against bacteria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

uniprot summary :

HP: Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes. Defects in HP are the cause of anhaptoglobinemia (AHP). AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Belongs to the peptidase S1 family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 16q22.2. Cellular Component: extracellular space; extracellular region. Molecular Function: antioxidant activity; protein binding; hemoglobin binding; catalytic activity. Biological Process: receptor-mediated endocytosis; response to hydrogen peroxide; metabolic process; immune system process; defense response to bacterium; negative regulation of oxidoreductase activity; acute-phase response; defense response. Disease: Anhaptoglobinemia

size1 :

48-Strip-Wells

price1 :

525 USD

size2 :

96-Strip-Wells

price2 :

905

more info or order :

MyBioSource product webpage