catalog number :
MBS280723
products type :
ELISA Kit
products full name :
Horse Brain-derived neurotrophic factor (BDNF) ELISA Kit
products short name :
Brain-derived neurotrophic factor
other names :
brain derived neurotrophic factor; Brain-derived neurotrophic factor; brain-derived neurotrophic factor; pre-pro-brain-derived; brain-derived neurotrophic factor
products gene name :
BDNF
other gene names :
BDNF; BDNF; BDNF
uniprot entry name :
BDNF_CHICK
specificity :
This assay has high sensitivity and excellent specificity for detection of Horse BDNF. No significant cross-reactivity or interference between Horse BDNF and analogues was observed.
storage stability :
Store at 2-8 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156 ng/mL - 10 ng/mL. Sensitivity: 0.078 ng/mL (mean of 6 independent assays)
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: For the quantitative detection of Horse Brain derived neurotrophic facor (BDNF) concentration in serum, plasma and other biological fluids. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate BDNF in Horse serum, plasma. An antibody specific for BDNF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BDNF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BDNF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BDNF bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AAZ31074.1
ncbi mol weight :
27,715 Da
ncbi pathways :
MAPK Signaling Pathway (199082); MAPK Signaling Pathway (83956); MAPK Signaling Pathway (456)