catalog number :
MBS280668
products type :
ELISA Kit
products full name :
Pig Epididymal secretory glutathione peroxidase (GPX5) ELISA Kit
products short name :
Epididymal secretory glutathione peroxidase
products gene name :
GPX5
specificity :
This assay has high sensitivity and excellent specificity for detection of Pig GPX5. No significant cross-reactivity or interference between Pig GPX5 and analogues was observed.
storage stability :
Store at 2-8 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.625 ng/mL - 40 ng/mL. Sensitivity: 0.31 ng/mL (mean of 6 independent assays)
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: For the quantitative detection of Pig Epididymal secretory glutathione peroxidase (GPX5) concentration in serum, plasma and other biological fluids. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitative GPX5 in Pig serum, plasma. An antibody specific for GPX5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPX5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for GPX5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPX5 bound in the initial step. The color development is stopped and the intensity of the color is measured.