ELISA/assay

Human Phosphoacetylglucosamine mutase (PGM3) ELISA Kit

MBS280407

48-Strip-Wells

405 USD

ELISA Kit

Human Phosphoacetylglucosamine mutase (PGM3) ELISA Kit

[Phosphoacetylglucosamine mutase]

[phosphoacetylglucosamine mutase isoform 1; Phosphoacetylglucosamine mutase; phosphoacetylglucosamine mutase; phosphoglucomutase 3; Acetylglucosamine phosphomutase]

[PGM3]

[PGM3; PGM3; AGM1; PAGM; IMD23; PGM 3; PGM 3]

Human

542

This assay has high sensitivity and excellent specificity for detection of Human PGM3. No significant cross-reactivity or interference between Human PGM3 and analogues was observed.

Store at 2-8 degree C.

Samples: Serum, Plasma, Other biological fluids. Assay Type: Sandwich

Sample Volume: 1-200 uL. Precision: Intra-assay Precision (Precision within an assay). Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays). Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Intra-Assay: CV. Inter-Assay: CV. Detection Wavelength: 450 nm

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PGM3 in samples. An antibody specific for PGM3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGM3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGM3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGM3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

315707006

NP_001186846.1

NM_001199917.1

O95394

62,941 Da

Amino Sugar And Nucleotide Sugar Metabolism Pathway (82979); Amino Sugar And Nucleotide Sugar Metabolism Pathway (350); Asparagine N-linked Glycosylation Pathway (161013); Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway (161014); Defective ALG1 Causes ALG1-CDG (CDG-1k) Pathway (1127605); Defective ALG11 Causes ALG11-CDG (CDG-1p) Pathway (1127608); Defective ALG12 Causes ALG12-CDG (CDG-1g) Pathway (1127601); Defective ALG14 Causes Congenital Myasthenic Syndrome (ALG14-CMS) Pathway (1127613); Defective ALG2 Causes ALG2-CDG (CDG-1i) Pathway (1127603); Defective ALG3 Causes ALG3-CDG (CDG-1d) Pathway (1127599)

This gene encodes a member of the phosphohexose mutase family. The encoded protein mediates both glycogen formation and utilization by catalyzing the interconversion of glucose-1-phosphate and glucose-6-phosphate. A non-synonymous single nucleotide polymorphism in this gene may play a role in resistance to diabetic nephropathy and neuropathy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

PGM3: Interconverts GlcNAc-6-P and GlcNAc-1-P. Belongs to the phosphohexose mutase family. Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 5.4.2.3; Isomerase. Chromosomal Location of Human Ortholog: 6q14.1. Cellular Component: cytosol. Molecular Function: magnesium ion binding; phosphoacetylglucosamine mutase activity; phosphoglucomutase activity. Biological Process: carbohydrate metabolic process; glucosamine metabolic process; glucose 1-phosphate metabolic process; hemopoiesis; protein N-linked glycosylation; protein O-linked glycosylation; spermatogenesis; UDP-N-acetylglucosamine biosynthetic process. Disease: Immunodeficiency 23

48-Strip-Wells

405 USD

96-Strip-Wells

675

5x96-Strip-Wells

3080

10x96-Strip-Wells

5805